PMID- 19389503
OWN - NLM
STAT- MEDLINE
DCOM- 20090505
LR  - 20090424
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 191
IP  - 1
DP  - 2009 May
TI  - Genomic amplification of the human telomerase RNA gene for differential diagnosis 
      of cervical disorders.
PG  - 10-6
LID - 10.1016/j.cancergencyto.2009.01.004 [doi]
AB  - To evaluate genomic amplification of the human telomerase RNA gene (TERC) as a 
      supportive approach to cytopathology or histopathology in diagnosis of low-grade 
      and high-grade uterine cervical lesions, 1,033 Chinese women at three medical 
      centers had liquid-based thin-layer cytopathologic examination and TERC detection 
      by fluorescence in situ hybridization (FISH). Human papillomavirus DNA testing, 
      colposcopy with or without biopsy, and histopathologic examination were conducted 
      as needed. In cytopathologic examination, genomic amplification of TERC was found 
      in 30 of 659 (4.6%) normal or benign cellular changes; in 23 of 170 (13.5%) 
      atypical squamous cells of undetermined significance (ASCUS); in 8 of 28 (28.6%) 
      atypical squamous cells with high-grade squamous intraepithelial lesion possible 
      (ASC-H); and in 26 of 103 (25.2%) low-grade (LSIL) and 64 of 73 (87.7%) 
      high-grade (HSIL) squamous intraepithelial lesions; with pairwise significant 
      difference (P< 0.05) in each, except ASC-H and LSIL (chi(2) = 0.127, P = 0.72). 
      In histopathologic examination, TERC was amplified in 28 of 671 (4.2%) normal, 
      inflammatory, or wart cases; in 17 of 233 (7.3%) cervical intraepithelial 
      neoplasia grade 1 cases (CIN 1); in 27 of 39 (69.2%) CIN 2 cases; in 57 of 67 
      (85.1%) CIN 3 cases; and in 22 of 23 (95.7%) cervical cancer cases; with pairwise 
      significant difference in each (P < 0.05). The number of cells with abnormal 
      signals increased and the abnormal signal patterns were diversified with 
      increasing severity of cervical dysplasia. FISH detection of TERC amplification 
      may provide an effective, noninvasive approach in conjunction with cytopathologic 
      or histopathologic evaluation for differential diagnosis of low- and high-grade 
      cervical disorders.
FAU - Tu, Zheng
AU  - Tu Z
AD  - Department of Gynecology, Peking University People's Hospital, No. 11 Xizhimen 
      South Street, Beijing 100044, China.
FAU - Zhang, Aipeng
AU  - Zhang A
FAU - Wu, Ruifang
AU  - Wu R
FAU - Jiang, Jing
AU  - Jiang J
FAU - Li, Yali
AU  - Li Y
FAU - Wulan, Na
AU  - Wulan N
FAU - Li, Jingran
AU  - Li J
FAU - Zhang, Yongmei
AU  - Zhang Y
FAU - Li, Yibing
AU  - Li Y
FAU - Chen, Zhong
AU  - Chen Z
FAU - Wei, Lihui
AU  - Wei L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Diagnosis, Differential
MH  - Female
MH  - *Gene Amplification
MH  - Genome, Human/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Papillomaviridae/genetics
MH  - RNA/*genetics
MH  - Telomerase/*genetics
MH  - Uterine Cervical Diseases/*diagnosis/enzymology/*genetics/pathology
EDAT- 2009/04/25 09:00
MHDA- 2009/05/06 09:00
CRDT- 2009/04/25 09:00
PHST- 2008/12/03 00:00 [received]
PHST- 2009/01/02 00:00 [revised]
PHST- 2009/01/05 00:00 [accepted]
PHST- 2009/04/25 09:00 [entrez]
PHST- 2009/04/25 09:00 [pubmed]
PHST- 2009/05/06 09:00 [medline]
AID - S0165-4608(09)00042-9 [pii]
AID - 10.1016/j.cancergencyto.2009.01.004 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2009 May;191(1):10-6. doi: 
      10.1016/j.cancergencyto.2009.01.004.