PMID- 19391039
OWN - NLM
STAT- MEDLINE
DCOM- 20090902
LR  - 20190116
IS  - 1029-2403 (Electronic)
IS  - 1026-8022 (Linking)
VI  - 50
IP  - 7
DP  - 2009 Jul
TI  - Dexamethasone and hypoxia upregulate CXCR4 expression in myeloma cells.
PG  - 1163-73
LID - 10.1080/10428190902893801 [doi]
AB  - We investigated the modulation of CXCR4 expression by cytokines, dexamethasone, 
      and hypoxia in myeloma cells in vitro. Tumor necrosis factor-alpha (TNF-alpha), 
      transforming growth factor-beta1 (TGF-beta1), and vascular endothelial growth 
      factor (VEGF) enhanced CXCR4 expression in RPMI8226 cells. In the myeloma cell 
      lines examined and primary bone marrow (BM) CD138+ cells, dexamethasone enhanced 
      CXCR4 expression both in the cytoplasm and on the cell surface, while 
      downregulating SDF-1 expression and secretion in BM stromal cells. Incubation of 
      cells under hypoxic conditions (1% O(2)) also induced upregulation of CXCR4 in 
      the cytoplasm and on the cell surface and enhanced chemotaxis in response to 
      stromal cell-derived factor-1 (SDF-1). Cell surface CXCR4 expression was more 
      prominent in annexin V-positive apoptotic cells. Given the roles of the 
      SDF-1/CXCR4 axis in the development and progression of myeloma, 
      CXCR4-downregulating agents may enhance the antitumor effects of dexamethasone.
FAU - Kim, Seong-Woo
AU  - Kim SW
AD  - Division of Hematology/Oncology, Department of Internal Medicine, College of 
      Medicine, Chungnam National University, Jung-gu, Daejeon, Korea.
FAU - Kim, Ha-Yon
AU  - Kim HY
FAU - Lee, Hyo-Jin
AU  - Lee HJ
FAU - Yun, Hwan-Jung
AU  - Yun HJ
FAU - Kim, Samyong
AU  - Kim S
FAU - Jo, Deog-Yeon
AU  - Jo DY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
RN  - 0 (Antineoplastic Agents, Hormonal)
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, CXCR4)
RN  - 0 (Syndecan-1)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
CIN - Leuk Lymphoma. 2009 Jul;50(7):1067-8. PMID: 19557626
MH  - Antineoplastic Agents, Hormonal/*pharmacology
MH  - Bone Marrow Cells/cytology
MH  - Cell Membrane/metabolism
MH  - Dexamethasone/*pharmacology
MH  - Flow Cytometry/methods
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - *Hypoxia
MH  - Interleukin-6/metabolism
MH  - Microscopy, Fluorescence/methods
MH  - Multiple Myeloma/*drug therapy/*metabolism
MH  - Receptors, CXCR4/metabolism/*physiology
MH  - Syndecan-1/biosynthesis
MH  - Transforming Growth Factor beta1/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2009/04/25 09:00
MHDA- 2009/09/03 06:00
CRDT- 2009/04/25 09:00
PHST- 2009/04/25 09:00 [entrez]
PHST- 2009/04/25 09:00 [pubmed]
PHST- 2009/09/03 06:00 [medline]
AID - 910664047 [pii]
AID - 10.1080/10428190902893801 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2009 Jul;50(7):1163-73. doi: 10.1080/10428190902893801.