PMID- 19392688
OWN - NLM
STAT- MEDLINE
DCOM- 20090921
LR  - 20220317
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 59
IP  - 3
DP  - 2009 Aug
TI  - Super-stretched pachytene chromosomes for fluorescence in situ hybridization 
      mapping and immunodetection of DNA methylation.
PG  - 509-16
LID - 10.1111/j.1365-313X.2009.03881.x [doi]
AB  - Meiotic pachytene chromosome-based fluorescence in situ hybridization (FISH) 
      mapping is one of the most important tools in plant molecular cytogenetic 
      research. Here we report a simple technique that allows stretching of pachytene 
      chromosomes of maize to up to at least 20 times their original size. A modified 
      Carnoy's II fixative (6:1:3 ethanol:chloroform:acetic acid) was used in the 
      procedure, and proved to be key for super-stretching of pachytene chromosomes. We 
      demonstrate that super-stretched pachytene chromosomes provide unprecedented 
      resolution for chromosome-based FISH mapping. DNA probes separated by as little 
      as 50 kb can be resolved on super-stretched chromosomes. A combination of FISH 
      with immunofluorescent detection of 5-methyl cytosine on super-stretched 
      pachytene chromosomes provides a powerful tool to reveal DNA methylation of 
      specific chromosomal domains, especially those associated with highly repetitive 
      DNA sequences.
FAU - Koo, Dal-Hoe
AU  - Koo DH
AD  - Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, 
      USA.
FAU - Jiang, Jiming
AU  - Jiang J
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090330
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (DNA, Plant)
RN  - 6R795CQT4H (5-Methylcytosine)
SB  - IM
MH  - 5-Methylcytosine
MH  - Chromosome Mapping/*methods
MH  - *Chromosomes, Plant
MH  - *DNA Methylation
MH  - DNA, Plant/genetics
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Zea mays/genetics
EDAT- 2009/04/28 09:00
MHDA- 2009/09/22 06:00
CRDT- 2009/04/28 09:00
PHST- 2009/04/28 09:00 [entrez]
PHST- 2009/04/28 09:00 [pubmed]
PHST- 2009/09/22 06:00 [medline]
AID - TPJ3881 [pii]
AID - 10.1111/j.1365-313X.2009.03881.x [doi]
PST - ppublish
SO  - Plant J. 2009 Aug;59(3):509-16. doi: 10.1111/j.1365-313X.2009.03881.x. Epub 2009 
      Mar 30.