PMID- 19394027
OWN - NLM
STAT- MEDLINE
DCOM- 20090701
LR  - 20191210
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1216
IP  - 22
DP  - 2009 May 29
TI  - Determination of thromboxanes, leukotrienes and lipoxins using high-temperature 
      capillary liquid chromatography-tandem mass spectrometry and on-line sample 
      preparation.
PG  - 4648-54
LID - 10.1016/j.chroma.2009.03.068 [doi]
AB  - An on-line strong cation-exchange (SCX)-reversed-phase (RP) capillary liquid 
      chromatographic (cLC) method with ion-trap tandem mass spectrometric (IT-MS/MS) 
      detection for the simultaneous determination of thromboxane (TX) B(2), TXB(3), 
      leukotriene (LT) B(4), LTD(4) and lipoxin (LX) A(4) in cell culture supernatants 
      was developed and validated. In the present method, a high temperature (70 
      degrees C) was used for the separation on the analytical column to obtain 
      efficient chromatography of the thromboxanes. An on-line sample preparation was 
      performed, where peptides/proteins contained in the matrix were removed by the 
      SCX column. Sample pre-treatment included dilution and filtration, and the 
      analysis time including all sample preparation steps was 60min per sample. Limits 
      of detection in the range of 1-4ng/mL cell culture supernatant, recoveries 
      between 30% and 100%, within day precisions of less than 20% RSD and between day 
      precisions of less than 30% RSD were obtained. Human mesenchymal stem cells 
      (hMSCs) were stimulated with cytokine-containing supernatants derived from 
      activated human T lymphocytes, and thromboxane, leukotriene and lipoxin 
      production was analysed using the developed method. TXB(2) was found in cultures 
      from both non-differentiated and differentiated hMSCs that were stimulated with a 
      cytokine-containing supernatant obtained from activated T-cells.
FAU - Dahl, Sandra Rinne
AU  - Dahl SR
AD  - Department of Chemistry, University of Oslo, Norway. sandrari@kjemi.uio.no
FAU - Kleiveland, Charlotte Ramstad
AU  - Kleiveland CR
FAU - Kassem, Moustapha
AU  - Kassem M
FAU - Lea, Tor
AU  - Lea T
FAU - Lundanes, Elsa
AU  - Lundanes E
FAU - Greibrokk, Tyge
AU  - Greibrokk T
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20090328
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Leukotrienes)
RN  - 0 (Lipoxins)
RN  - 0 (Thromboxanes)
SB  - IM
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chromatography, Liquid/instrumentation/*methods
MH  - Humans
MH  - Leukocytes, Mononuclear/chemistry/metabolism
MH  - Leukotrienes/*analysis/metabolism
MH  - Lipoxins/*analysis/metabolism
MH  - Online Systems
MH  - Tandem Mass Spectrometry/*methods
MH  - Thromboxanes/*analysis/metabolism
EDAT- 2009/04/28 09:00
MHDA- 2009/07/02 09:00
CRDT- 2009/04/28 09:00
PHST- 2009/01/09 00:00 [received]
PHST- 2009/03/23 00:00 [revised]
PHST- 2009/03/24 00:00 [accepted]
PHST- 2009/04/28 09:00 [entrez]
PHST- 2009/04/28 09:00 [pubmed]
PHST- 2009/07/02 09:00 [medline]
AID - S0021-9673(09)00475-0 [pii]
AID - 10.1016/j.chroma.2009.03.068 [doi]
PST - ppublish
SO  - J Chromatogr A. 2009 May 29;1216(22):4648-54. doi: 10.1016/j.chroma.2009.03.068. 
      Epub 2009 Mar 28.