PMID- 19394592
OWN - NLM
STAT- MEDLINE
DCOM- 20090612
LR  - 20131121
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 258
IP  - 1
DP  - 2009
TI  - Human monocytes but not dendritic cells are killed by blocking of autocrine 
      cyclooxygenase activity.
PG  - 107-14
LID - 10.1016/j.cellimm.2009.03.019 [doi]
AB  - Dendritic cells (DCs), in peripheral tissues, derive mostly from blood precursors 
      that differentiate into DCs under the influence of the local microenvironment. 
      Monocytes constitute the main known DC precursors in blood and their infiltration 
      into tissues is up-regulated during inflammation. During this process, the local 
      production of mediators, like prostaglandins (PGs), influence significantly DC 
      differentiation and function. In the present paper we show that treatment of 
      blood adherent mononuclear cells with 10microM indomethacin, a dose achieved in 
      human therapeutic settings, causes monocytes' progressive death but does not 
      affect DCs viability or cell surface phenotype. This resistance of DCs was 
      observed both for cells differentiated in vitro from blood monocytes and for a 
      population with DCs characteristics already present in blood. This phenomenon 
      could affect the local balance of antigen-presenting cells, influence the 
      induction and pattern of immune responses developed under the treatment with 
      non-steroidal anti-inflammatory drugs and, therefore, deserves further 
      investigation.
FAU - Oliveira, Bruno L
AU  - Oliveira BL
AD  - Laboratory of Tumor Immunology, Department of Immunology, University of Sao 
      Paulo, Sao Paulo, Brazil.
FAU - Cavalcanti, Clara M
AU  - Cavalcanti CM
FAU - Azevedo, Ana Paula S
AU  - Azevedo AP
FAU - Tomiyoshi, Marcio Y
AU  - Tomiyoshi MY
FAU - Bergami-Santos, Patricia C
AU  - Bergami-Santos PC
FAU - Barbuto, Jose Alexandre M
AU  - Barbuto JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090425
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Cyclooxygenase Inhibitors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
MH  - Cell Death
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - Cyclooxygenase Inhibitors/*pharmacology
MH  - Dendritic Cells/*drug effects/enzymology/immunology
MH  - Humans
MH  - Indomethacin/*pharmacology
MH  - Monocytes/*drug effects/enzymology/immunology
MH  - Prostaglandin-Endoperoxide Synthases/*drug effects
MH  - Tumor Necrosis Factor-alpha/immunology/metabolism
EDAT- 2009/04/28 09:00
MHDA- 2009/06/13 09:00
CRDT- 2009/04/28 09:00
PHST- 2008/08/06 00:00 [received]
PHST- 2009/03/26 00:00 [revised]
PHST- 2009/03/30 00:00 [accepted]
PHST- 2009/04/28 09:00 [entrez]
PHST- 2009/04/28 09:00 [pubmed]
PHST- 2009/06/13 09:00 [medline]
AID - S0008-8749(09)00059-8 [pii]
AID - 10.1016/j.cellimm.2009.03.019 [doi]
PST - ppublish
SO  - Cell Immunol. 2009;258(1):107-14. doi: 10.1016/j.cellimm.2009.03.019. Epub 2009 
      Apr 25.