PMID- 1939580
OWN - NLM
STAT- MEDLINE
DCOM- 19911223
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 29
IP  - 10
DP  - 1991 Oct
TI  - Synthetic peptide-based immunoassays for distinguishing between human T-cell 
      lymphotropic virus type I and type II infections in seropositive individuals.
PG  - 2253-8
AB  - Until now, serologic tests that distinguish the closely related human T-cell 
      lymphotropic virus types I (HTLV-I) and II (HTLV-II) infections have not been 
      available. Synthetic peptide assays, employing peptides derived from the core and 
      envelope proteins of HTLV-I and HTLV-II (SynthEIA and Select-HTLV tests), were 
      evaluated for the ability to serologically discriminate HTLV-I and HTLV-II 
      infections. Of 32 HTLV-I- and 57 HTLV-II-positive serum specimens from 
      individuals whose infections were confirmed by polymerase chain reaction, the 
      SynthEIA test categorized 29 (91%) as HTLV-I and 50 (88%) as HTLV-II, and 10 
      (11%) were nontypeable. In contrast, the Select-HTLV test categorized 32 (100%) 
      as HTLV-I and 55 (96%) as HTLV-II, and 2 (2%) were nontypeable. The specificity 
      of both the assays in seropositive serum specimens was 100% in that none of the 
      specimens were incorrectly classified. Additional serum specimens obtained from 
      clinically diseased patients from the United States (n = 8) and asymptomatic 
      carriers and patients from Japan (an endemic population for HTLV-I; n = 40) were 
      categorized as HTLV-I by at least one of the assays, while serum specimens from 
      Guaymi Indians from Panama (an endemic population for HTLV-II; n = 13) were 
      categorized as HTLV-II. Thus, peptide enzyme immunoassays appear to represent a 
      simple technique employing chemically synthesized antigens for discrimination 
      between antibodies of HTLV-I and HTLV-II.
FAU - Lal, R B
AU  - Lal RB
AD  - Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
FAU - Heneine, W
AU  - Heneine W
FAU - Rudolph, D L
AU  - Rudolph DL
FAU - Present, W B
AU  - Present WB
FAU - Hofhienz, D
AU  - Hofhienz D
FAU - Hartley, T M
AU  - Hartley TM
FAU - Khabbaz, R F
AU  - Khabbaz RF
FAU - Kaplan, J E
AU  - Kaplan JE
LA  - eng
SI  - GENBANK/J02029
SI  - GENBANK/M10060
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Peptides)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Antibodies, Viral/blood
MH  - DNA, Viral/genetics
MH  - Evaluation Studies as Topic
MH  - Female
MH  - HTLV-I Infections/*diagnosis/immunology
MH  - HTLV-II Infections/*diagnosis/immunology
MH  - Human T-lymphotropic virus 1/genetics/immunology
MH  - Human T-lymphotropic virus 2/genetics/immunology
MH  - Humans
MH  - Immunoassay/*methods
MH  - Male
MH  - Molecular Sequence Data
MH  - Peptides/immunology
MH  - Viral Proteins/analysis/*immunology
PMC - PMC270308
EDAT- 1991/10/01 00:00
MHDA- 1991/10/01 00:01
PMCR- 1991/10/01
CRDT- 1991/10/01 00:00
PHST- 1991/10/01 00:00 [pubmed]
PHST- 1991/10/01 00:01 [medline]
PHST- 1991/10/01 00:00 [entrez]
PHST- 1991/10/01 00:00 [pmc-release]
AID - 10.1128/jcm.29.10.2253-2258.1991 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1991 Oct;29(10):2253-8. doi: 10.1128/jcm.29.10.2253-2258.1991.