PMID- 1939596
OWN - NLM
STAT- MEDLINE
DCOM- 19911223
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 29
IP  - 10
DP  - 1991 Oct
TI  - Detection and differentiation of antibodies to human T-cell lymphotropic virus 
      types I and II by the immunofluorescence method.
PG  - 2345-7
AB  - We compared the sensitivities of the prototype human T-cell lymphotropic virus 
      type I (HTLV-I)- and HTLV-II-transformed cell lines, MT2 and Mo-T, with that of 
      an HTLV-II-infected cell line, clone 19, established in our laboratory, in the 
      immunofluorescence (IF) test for detection of antibody to HTLV-I and HTLV-II. In 
      addition, IF antibody titers with the three antigens were determined, and the 
      results were compared with HTLV-I and HTLV-II typing by polymerase chain reaction 
      (PCR). The MT2 cell line was more sensitive than the two HTLV-II cell lines for 
      detecting HTLV-I antibody by IF, and clone 19 was more sensitive than Mo-T or MT2 
      for measuring HTLV-II antibody. In the titration study, the antigen that gave the 
      highest titer correlated completely with the HTLV type determined by PCR, 
      indicating that the relatively simple IF titration method can be used for 
      differentiating HTLV-I and HTLV-II antibody in sera and plasmas.
FAU - Gallo, D
AU  - Gallo D
AD  - Viral and Rickettsial Disease Laboratory, State of California Department of 
      Health Services, Berkeley 94704.
FAU - Penning, L M
AU  - Penning LM
FAU - Hanson, C V
AU  - Hanson CV
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (HTLV-I Antigens)
RN  - 0 (HTLV-II Antibodies)
RN  - 0 (HTLV-II Antigens)
SB  - IM
MH  - Cell Line, Transformed
MH  - Evaluation Studies as Topic
MH  - *Fluorescent Antibody Technique
MH  - HTLV-I Antibodies/*blood
MH  - HTLV-I Antigens
MH  - HTLV-II Antibodies/*blood
MH  - HTLV-II Antigens
MH  - Human T-lymphotropic virus 1/classification/genetics/immunology
MH  - Human T-lymphotropic virus 2/classification/genetics/immunology
MH  - Humans
MH  - Polymerase Chain Reaction
PMC - PMC270328
EDAT- 1991/10/01 00:00
MHDA- 1991/10/01 00:01
PMCR- 1991/10/01
CRDT- 1991/10/01 00:00
PHST- 1991/10/01 00:00 [pubmed]
PHST- 1991/10/01 00:01 [medline]
PHST- 1991/10/01 00:00 [entrez]
PHST- 1991/10/01 00:00 [pmc-release]
AID - 10.1128/jcm.29.10.2345-2347.1991 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1991 Oct;29(10):2345-7. doi: 10.1128/jcm.29.10.2345-2347.1991.