PMID- 1939646
OWN - NLM
STAT- MEDLINE
DCOM- 19911213
LR  - 20181113
IS  - 0021-9738 (Print)
IS  - 0021-9738 (Linking)
VI  - 88
IP  - 5
DP  - 1991 Nov
TI  - Regulation of transforming growth factor-beta 1 gene expression by 
      glucocorticoids in normal human T lymphocytes.
PG  - 1574-80
AB  - Glucocorticoids (GC) modulate immune function in a number of ways, including 
      suppression of T cell proliferation and other IL-2-mediated T cell functions. 
      These inhibitory effects are similar to those induced by transforming growth 
      factor-beta 1 (TGF-beta 1), a cytokine with potent T cell inhibiting activities. 
      We examined the hypothesis that GC effects may be at least partially achieved 
      through modulation of the expression of the TGF-beta 1 gene in activated T cells. 
      Normal T cells were cultured with or without purified phytohemagglutinin (PHA-p) 
      and 4 beta-phorbol 12-myristate 13-acetate (PMA) in the presence or absence of 
      the synthetic GC, dexamethasone (100-200 micrograms/ml). The production of latent 
      and active forms of TGF beta by these cells were analyzed by immunoblotting and 
      bioassays. The steady-state levels of TGF-beta 1 mRNA were analyzed in total RNA 
      from these cells by Northern hybridizations using a human TGF-beta 1 cDNA. The 
      results showed that dexamethasone caused an increase in TGF beta production and a 
      dose-dependent two to fourfold increase in TGF-beta 1 mRNA in activated as well 
      as in unstimulated T cells, 1 h after exposure of the cultures to the steroid. 
      The increase in TGF-beta 1 mRNA levels by dexamethasone was further potentiated 
      two to threefold by cycloheximide, suggesting that the steroid effect may be due 
      to inhibition of the synthesis of proteins that decrease TGF-beta 1 gene 
      transcription or the stability of its transcripts. Finally, in vitro nuclear 
      transcription studies indicated the dexamethasone effects on TGF-beta 1 gene 
      expression to be largely transcriptional.
FAU - AyanlarBatuman, O
AU  - AyanlarBatuman O
AD  - Cardeza Foundation for Hematologic Research, Jefferson Medical College, Thomas 
      Jefferson University, Philadelphia, Pennsylvania 19107.
FAU - Ferrero, A P
AU  - Ferrero AP
FAU - Diaz, A
AU  - Diaz A
FAU - Jimenez, S A
AU  - Jimenez SA
LA  - eng
GR  - AM 19101/AM/NIADDK NIH HHS/United States
GR  - HL41214/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Invest
JT  - The Journal of clinical investigation
JID - 7802877
RN  - 0 (Transforming Growth Factor beta)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 98600C0908 (Cycloheximide)
SB  - IM
MH  - Cells, Cultured
MH  - Cycloheximide/pharmacology
MH  - Dexamethasone/*pharmacology
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - Lymphocyte Activation
MH  - T-Lymphocytes/*metabolism
MH  - Transcription, Genetic/drug effects
MH  - Transforming Growth Factor beta/biosynthesis/*genetics
PMC - PMC295675
EDAT- 1991/11/01 00:00
MHDA- 1991/11/01 00:01
PMCR- 1991/11/01
CRDT- 1991/11/01 00:00
PHST- 1991/11/01 00:00 [pubmed]
PHST- 1991/11/01 00:01 [medline]
PHST- 1991/11/01 00:00 [entrez]
PHST- 1991/11/01 00:00 [pmc-release]
AID - 10.1172/JCI115469 [doi]
PST - ppublish
SO  - J Clin Invest. 1991 Nov;88(5):1574-80. doi: 10.1172/JCI115469.