PMID- 19396577
OWN - NLM
STAT- MEDLINE
DCOM- 20090512
LR  - 20220110
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 154
IP  - 5
DP  - 2009
TI  - Characterization of monoclonal antibodies against the nonstructural 5A protein of 
      hepatitis C virus.
PG  - 843-51
LID - 10.1007/s00705-009-0386-9 [doi]
AB  - The nonstructural 5A (NS5A) protein of hepatitis C virus (HCV) is a 
      multifunctional protein that leads to pleiotropic responses, in part by 
      regulating cell growth and cellular signaling pathways. Here, we produced 
      monoclonal antibodies (mAbs) directed against the HCV NS5A protein. The 
      N-terminal epitope was mapped to amino acids 60-80 of the NS5A protein, and the 
      epitope in the middle region was mapped to amino acids 221-236. Because these 
      epitopes overlap with binding regions of human vesicle-associated 
      membrane-protein-associated protein (hVAP)-B and TNF-receptor-associated factor 2 
      (TRAF2), respectively, we investigated these mAbs for their potential capacity to 
      inhibit viral and cellular interactions. We found that NS5A and hVAP-B 
      interaction was abolished by mAb E5D3, and NS5A and TRAF2 interaction was 
      inhibited by mAb C6D4. Since hVAP-B is necessary for HCV replication and TRAF2 is 
      the major transducer in TNF signaling cascades, these data may provide further 
      insights into the mechanisms underlying HCV replication and viral modulation of 
      host signal transduction.
FAU - Kang, Sang Min
AU  - Kang SM
AD  - National Research Laboratory of Hepatitis C Virus, Ilsong Institute of Life 
      Science, Hallym University, 1605-4 Gwanyang-dong, Dongan-gu, Anyang 431-060, 
      Korea.
FAU - Jun, Hyun-Jung
AU  - Jun HJ
FAU - Lim, Yun-Sook
AU  - Lim YS
FAU - Choi, Soo-Ho
AU  - Choi SH
FAU - Hwang, Soon B
AU  - Hwang SB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090426
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Epitopes)
RN  - 0 (TNF Receptor-Associated Factor 2)
RN  - 0 (VAPB protein, human)
RN  - 0 (Vesicular Transport Proteins)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 2.7.7.48 (NS-5 protein, hepatitis C virus)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*metabolism
MH  - Cell Line
MH  - *Epitope Mapping
MH  - Epitopes/immunology
MH  - Female
MH  - Hepacivirus/*physiology
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - TNF Receptor-Associated Factor 2/metabolism
MH  - Vesicular Transport Proteins/metabolism
MH  - Viral Nonstructural Proteins/isolation & purification/*metabolism
EDAT- 2009/04/28 09:00
MHDA- 2009/05/13 09:00
CRDT- 2009/04/28 09:00
PHST- 2008/12/01 00:00 [received]
PHST- 2009/04/16 00:00 [accepted]
PHST- 2009/04/28 09:00 [entrez]
PHST- 2009/04/28 09:00 [pubmed]
PHST- 2009/05/13 09:00 [medline]
AID - 10.1007/s00705-009-0386-9 [doi]
PST - ppublish
SO  - Arch Virol. 2009;154(5):843-51. doi: 10.1007/s00705-009-0386-9. Epub 2009 Apr 26.