PMID- 19396950
OWN - NLM
STAT- MEDLINE
DCOM- 20090522
LR  - 20191111
IS  - 1462-2920 (Electronic)
IS  - 1462-2912 (Linking)
VI  - 11
IP  - 4
DP  - 2009 Apr
TI  - 'Candidatus Lumbricincola', a novel lineage of uncultured Mollicutes from 
      earthworms of family Lumbricidae.
PG  - 1016-26
AB  - Molecular signatures of new, as yet uncultured mollicute-like organisms (MLOs) 
      have been detected in total rRNA and DNA extracted from tissues, gut contents and 
      casts of four species of the earthworm family Lumbricidae. The MLO 16S rRNA 
      sequences exhibited low identity to those of known Mollicutes species and formed 
      a monophyletic cluster distantly affiliated to the 'Candidatus Bacilloplasma' 
      (84.9%) and almost equidistant to the other main phylogenetic group of Mollicutes 
      (< 79.8%) and the classes Bacilli (< 79.5%) and Clostridia (< 76.1%). SSCP 
      profiling and sequence analysis of bands and bacterial clones derived from the 
      earthworms and substrata revealed high phylogenetic relatedness of MLOs in 
      earthworms from different geographic locations (Russia and Germany), with no 
      obvious host species specificity being observed. Fluorescence in situ 
      hybridization (FISH) analysis with a nucleotide probe specific for the new MLO 
      group localized them to the coelomic fluids of earthworms. A new taxonomic group 
      within the Mollicutes, designated 'Candidatus Lumbricincola', is proposed to 
      include these as yet uncultured organisms.
FAU - Nechitaylo, Taras Y
AU  - Nechitaylo TY
AD  - Environmental Microbiology Laboratory, Helmholtz Centre for Infection Research, 
      38124 Braunschweig, Germany.
FAU - Timmis, Kenneth N
AU  - Timmis KN
FAU - Golyshin, Peter N
AU  - Golyshin PN
LA  - eng
SI  - GENBANK/AM905473
SI  - GENBANK/AM905474
SI  - GENBANK/AM905475
SI  - GENBANK/AM905476
SI  - GENBANK/AM905477
SI  - GENBANK/AM905478
SI  - GENBANK/AM905479
SI  - GENBANK/AM905480
SI  - GENBANK/AM905481
SI  - GENBANK/AM905482
SI  - GENBANK/AM905483
SI  - GENBANK/AM905484
SI  - GENBANK/AM905485
SI  - GENBANK/AM905486
SI  - GENBANK/AM905487
SI  - GENBANK/AM905488
SI  - GENBANK/AM905489
SI  - GENBANK/AM905490
SI  - GENBANK/AM905491
SI  - GENBANK/AM905492
SI  - GENBANK/AM905493
SI  - GENBANK/AM905494
SI  - GENBANK/AM905495
SI  - GENBANK/AM905496
SI  - GENBANK/AM905497
SI  - GENBANK/AM905498
SI  - GENBANK/AM905499
SI  - GENBANK/AM905500
SI  - GENBANK/AM905501
SI  - GENBANK/AM905502
SI  - GENBANK/AM905503
SI  - GENBANK/AM905504
SI  - GENBANK/AM905505
SI  - GENBANK/AM905506
SI  - GENBANK/AM905507
SI  - GENBANK/AM905508
SI  - GENBANK/AM905509
SI  - GENBANK/AM905510
SI  - GENBANK/AM905511
SI  - GENBANK/AM905512
SI  - GENBANK/AM905513
SI  - GENBANK/AM905514
SI  - GENBANK/AM905515
SI  - GENBANK/AM905516
SI  - GENBANK/AM905517
SI  - GENBANK/AM905518
SI  - GENBANK/AM905519
SI  - GENBANK/AM905520
SI  - GENBANK/AM905521
SI  - GENBANK/AM905522
SI  - GENBANK/AM905523
SI  - GENBANK/AM905524
SI  - GENBANK/AM905525
SI  - GENBANK/AM905526
SI  - GENBANK/AM905527
SI  - GENBANK/AM905528
SI  - GENBANK/AM905529
SI  - GENBANK/AM905530
SI  - GENBANK/AM905531
SI  - GENBANK/AM905532
SI  - GENBANK/AM905533
SI  - GENBANK/AM905534
SI  - GENBANK/AM905535
SI  - GENBANK/AM905536
SI  - GENBANK/AM905537
SI  - GENBANK/AM905538
SI  - GENBANK/AM905539
SI  - GENBANK/AM905540
SI  - GENBANK/AM905541
SI  - GENBANK/AM905542
SI  - GENBANK/AM905543
SI  - GENBANK/AM905544
SI  - GENBANK/AM905545
SI  - GENBANK/AM905546
SI  - GENBANK/AM905547
SI  - GENBANK/AM905548
SI  - GENBANK/AM905549
SI  - GENBANK/AM905550
SI  - GENBANK/AM905551
SI  - GENBANK/AM905552
SI  - GENBANK/AM905553
SI  - GENBANK/AM905554
SI  - GENBANK/AM905555
SI  - GENBANK/AM905556
SI  - GENBANK/AM905557
SI  - GENBANK/FM165581
SI  - GENBANK/FM165582
SI  - GENBANK/FM165583
SI  - GENBANK/FM165584
SI  - GENBANK/FM165585
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Cluster Analysis
MH  - DNA Fingerprinting
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Genes, rRNA
MH  - Germany
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Oligochaeta/*microbiology
MH  - Phylogeny
MH  - RNA, Bacterial/genetics
MH  - RNA, Ribosomal, 16S/genetics
MH  - Russia
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Nucleic Acid
MH  - Tenericutes/*classification/*isolation & purification
EDAT- 2009/04/28 09:00
MHDA- 2009/05/23 09:00
CRDT- 2009/04/28 09:00
PHST- 2009/04/28 09:00 [entrez]
PHST- 2009/04/28 09:00 [pubmed]
PHST- 2009/05/23 09:00 [medline]
AID - 10.1111/j.1462-2920.2008.01837.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2009 Apr;11(4):1016-26. doi: 10.1111/j.1462-2920.2008.01837.x.