PMID- 19397832
OWN - NLM
STAT- MEDLINE
DCOM- 20090806
LR  - 20211020
IS  - 1477-7827 (Electronic)
IS  - 1477-7827 (Linking)
VI  - 7
DP  - 2009 Apr 28
TI  - Host immune responses to chlamydial inclusion membrane proteins B and C in 
      Chlamydia trachomatis infected women with or without fertility disorders.
PG  - 38
LID - 10.1186/1477-7827-7-38 [doi]
AB  - BACKGROUND: With an increase in the number of putative inclusion membrane 
      proteins (incs) in chlamydial genomes, there is a need for understanding their 
      contribution in host-pathogen interactions. Thus in this study we determined the 
      host mucosal and peripheral immune responses to incs (IncB and IncC) of Chlamydia 
      trachomatis (CT). METHODS: Female patients (n = 296) attending the gynaecology 
      out patient department of Safdarjung hospital, New Delhi were enrolled for the 
      study and were clinically characterized into two groups; CT-positive fertile 
      women (n = 38) and CT-positive women with fertility disorders (n = 29). 
      Uninfected healthy fertile women were enrolled as controls (n = 31). Gene 
      specific PCRs were used for detection of incB and incC genes in endocervical 
      samples of CT-positive patients. ELISA and Western blot assay were used for 
      detection of IgA and IgG antibodies to IncB and IncC in cervical washes and sera. 
      Effect of IncB and IncC stimulation of cervical cells and PBMCs on cellular 
      proliferation and cytotoxity was determined using MTT assay and Lactate 
      dehydrogenase (LDH)-cytotoxicity assay respectively. Modulation of cytokines 
      (Interleukin (IL)-1 Beta, IL-4, IL-5, IL-6, IL-10, Interferon-gamma, IL-12, Tumor 
      Necrosis Factor-alpha and Granulocyte macrophage colony-stimulating factor 
      (GM-CSF)) in cervical cells and PBMCs upon stimulation with IncB and IncC was 
      determined by real-time reverse-transcriptase (RT)-PCR and ELISA. Further, CD4 
      positive T cells were purified from cervical cells and peripheral blood 
      mononuclear cells (PBMCs) and secreted cytokines (Interferon-gamma and IL-4) were 
      evaluated by ELISPOT and real-time RT-PCR. RESULTS: Using MTT assay, 
      significantly high proliferative responses (P < 0.05) were observed in 
      inc-stimulated cervical cells and PBMCs from CT-positive fertile women compared 
      to CT-positive women with fertility disorders and controls. Interferon-gamma, 
      IL-12 and GM-CSF were found to be elevated in inc-stimulated cervical cells and 
      PBMCs of CT-positive fertile women compared to CT-positive women with fertility 
      disorders and controls (P < 0.05). In contrast, IL-1 Beta, IL-4, IL-5, IL-6 and 
      IL-10 levels were found to be higher in CT-positive women with fertility 
      disorders compared to CT-positive fertile women and controls (P < 0.05). 
      Interferon-gamma secreting cells and mRNA expression in inc-stimulated cervical 
      and peripheral CD4 positive T cells were significantly higher (P < 0.05) in CT 
      positive fertile women compared to CT-positive women with fertility disorders. 
      CONCLUSION: Our data overall suggests that CT incs, IncB and IncC modulate host 
      immune responses and may have a role in protection/pathogenesis of genital 
      chlamydial infection in women.
FAU - Gupta, Rishein
AU  - Gupta R
AD  - Institute of Pathology-ICMR, Safdarjang Hospital Campus, New Delhi, India. 
      rishein9@rediffmail.com
FAU - Srivastava, Pragya
AU  - Srivastava P
FAU - Vardhan, Harsh
AU  - Vardhan H
FAU - Salhan, Sudha
AU  - Salhan S
FAU - Mittal, Aruna
AU  - Mittal A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090428
PL  - England
TA  - Reprod Biol Endocrinol
JT  - Reproductive biology and endocrinology : RB&E
JID - 101153627
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Cytokines)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - Antibodies, Bacterial/immunology
MH  - CD4-Positive T-Lymphocytes/cytology/immunology/microbiology
MH  - Cell Division/immunology
MH  - Cells, Cultured
MH  - Cervix Uteri/cytology/immunology/microbiology
MH  - Chlamydia Infections/epidemiology/*immunology/*microbiology
MH  - Chlamydia trachomatis/genetics/*immunology/pathogenicity
MH  - Cytokines/genetics/immunology
MH  - Female
MH  - Gene Expression Regulation, Bacterial/immunology
MH  - Humans
MH  - Infertility, Female/immunology/*microbiology
MH  - Leukocytes, Mononuclear/cytology/immunology/*microbiology
MH  - Membrane Proteins/genetics/*immunology
MH  - Polymerase Chain Reaction
MH  - Seroepidemiologic Studies
MH  - Virulence
PMC - PMC2695819
EDAT- 2009/04/29 09:00
MHDA- 2009/08/07 09:00
PMCR- 2009/04/28
CRDT- 2009/04/29 09:00
PHST- 2009/02/16 00:00 [received]
PHST- 2009/04/28 00:00 [accepted]
PHST- 2009/04/29 09:00 [entrez]
PHST- 2009/04/29 09:00 [pubmed]
PHST- 2009/08/07 09:00 [medline]
PHST- 2009/04/28 00:00 [pmc-release]
AID - 1477-7827-7-38 [pii]
AID - 10.1186/1477-7827-7-38 [doi]
PST - epublish
SO  - Reprod Biol Endocrinol. 2009 Apr 28;7:38. doi: 10.1186/1477-7827-7-38.