PMID- 19399393
OWN - NLM
STAT- MEDLINE
DCOM- 20110317
LR  - 20211020
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 29
IP  - 2
DP  - 2009 Apr
TI  - The effect of brain-derived neurotrophic factor on angiogenesis.
PG  - 139-43
LID - 10.1007/s11596-009-0201-6 [doi]
AB  - To investigate the in vitro and in vivo proangiogenic effects of brain-derived 
      neurotrophic factor (BDNF), human umbilical vein endothelial cells (HUVECs) were 
      isolated and cultured in primary culture. The effect of BDNF on the proliferation 
      of HUVECs was examined by MTT assay. The effects of BDNF on HUVEC migration and 
      tube formation were studied by modified Boyden chamber assay and tube formation 
      assay, respectively. Matrigel plug assay and chorioallantoic membrane assay were 
      used to evaluate the effects of BDNF on angiogenesis in vivo. Our results showed 
      that BDNF substantially stimulated the migration and tube formation of HUVECs in 
      vitro, although it did not induce HUVEC proliferation. BDNF also induced 
      angiogenesis both in matrigel plug of mouse model and in chick chorioallantoic 
      membrane. In conclusion, BDNF can promote angiogenesis both in vitro and in vivo, 
      and may be a proangiogenic factor.
FAU - Sun, Chunyan
AU  - Sun C
AD  - Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, 430022, China. ayan0618@163.com
FAU - Hu, Yu
AU  - Hu Y
FAU - Chu, Zhangbo
AU  - Chu Z
FAU - Huang, Jing
AU  - Huang J
FAU - Zhang, Lu
AU  - Zhang L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090428
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (Angiogenesis Inducing Agents)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Angiogenesis Inducing Agents/*pharmacology
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Chick Embryo
MH  - Endothelial Cells/*cytology/drug effects
MH  - Female
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neovascularization, Physiologic/*drug effects
MH  - Umbilical Veins/cytology
MH  - Vascular Endothelial Growth Factor A/*metabolism
EDAT- 2009/04/29 09:00
MHDA- 2011/03/18 06:00
CRDT- 2009/04/29 09:00
PHST- 2008/10/03 00:00 [received]
PHST- 2009/04/29 09:00 [entrez]
PHST- 2009/04/29 09:00 [pubmed]
PHST- 2011/03/18 06:00 [medline]
AID - 10.1007/s11596-009-0201-6 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2009 Apr;29(2):139-43. doi: 
      10.1007/s11596-009-0201-6. Epub 2009 Apr 28.