PMID- 19401162
OWN - NLM
STAT- MEDLINE
DCOM- 20090707
LR  - 20220318
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1262
DP  - 2009 Mar 25
TI  - Expression of monocyte chemoattractant protein-1 in the cerebral artery after 
      experimental subarachnoid hemorrhage.
PG  - 73-80
LID - 10.1016/j.brainres.2009.01.017 [doi]
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a potent chemokine that plays an 
      important role in the recruitment of macrophages. Although previous studies have 
      demonstrated that MCP-1 has been shown to be involved in the damaging 
      inflammatory processes associated with stroke, infection, neoplasia, and others 
      in the central nervous system, the role of MCP-1 in the cerebral artery after 
      experimental subarachnoid hemorrhage (SAH) in rats has been largely unexplored. 
      This study was undertaken to investigate the expression of the MCP-1 in SAH model 
      and to clarify the potential role of MCP-1 in cerebral vasospasm. A total of 80 
      rats were randomly divided into four groups: control group; day 3, day 5, and day 
      7 groups. Day 3, day 5, and day 7 groups were all SAH groups. The animals in day 
      3, day 5 and day 7 groups were subjected to injection of autologous blood into 
      cisterna magna twice on day 0 and day 2 and were killed on days 3, 5, and 7, 
      respectively. Cross-sectional area of basilar artery was measured and the MCP-1 
      expression was assessed by real-time PCR, Western blot and immunohistochemistry. 
      The cross-sectional area of basilar artery was found to be 85,373+/-8794 mum(2) 
      in control group, 59,210+/-7281 mum(2) in day 3, 50,536+/-6519 mum(2) in day 5, 
      and 66,360+/-7452 mum(2) in day 7, respectively. The basilar arteries exhibited 
      vasospasm after SAH and became more severe on day 5. The elevated mRNA and 
      protein of MCP-1 were detected after SAH and peaked on day 5. MCP-1 is 
      increasingly expressed in a parallel time course to the development of cerebral 
      vasospasm in a rat experimental model of SAH and these findings might have 
      important implications during the administration of specific MCP-1 antagonists in 
      order to prevent or reduce cerebral vasospasm caused by SAH.
FAU - Lu, Hua
AU  - Lu H
AD  - Department of Neurosurgery, Jinling Hospital, Clinical School of Nanjing 
      University, 305 East Zhongshan Road, Nanjing 210002, China.
FAU - Shi, Ji-Xin
AU  - Shi JX
FAU - Chen, Hui-Lin
AU  - Chen HL
FAU - Hang, Chun-Hua
AU  - Hang CH
FAU - Wang, Han-Dong
AU  - Wang HD
FAU - Yin, Hong-Xia
AU  - Yin HX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090121
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Basilar Artery/*physiology
MH  - Cerebrovascular Circulation/physiology
MH  - Chemokine CCL2/*genetics/*metabolism
MH  - Disease Models, Animal
MH  - Immunohistochemistry
MH  - Male
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Subarachnoid Hemorrhage/immunology/metabolism/physiopathology
MH  - *Vasospasm, Intracranial/immunology/metabolism/physiopathology
EDAT- 2009/04/30 09:00
MHDA- 2009/07/08 09:00
CRDT- 2009/04/30 09:00
PHST- 2008/09/22 00:00 [received]
PHST- 2009/01/01 00:00 [revised]
PHST- 2009/01/05 00:00 [accepted]
PHST- 2009/04/30 09:00 [entrez]
PHST- 2009/04/30 09:00 [pubmed]
PHST- 2009/07/08 09:00 [medline]
AID - S0006-8993(09)00048-1 [pii]
AID - 10.1016/j.brainres.2009.01.017 [doi]
PST - ppublish
SO  - Brain Res. 2009 Mar 25;1262:73-80. doi: 10.1016/j.brainres.2009.01.017. Epub 2009 
      Jan 21.