PMID- 19401617
OWN - NLM
STAT- MEDLINE
DCOM- 20090702
LR  - 20191210
IS  - 1423-0100 (Electronic)
IS  - 0300-5526 (Linking)
VI  - 52
IP  - 2
DP  - 2009
TI  - Rapid detection of porcine reproductive and respiratory syndrome virus by reverse 
      transcription loop-mediated isothermal amplification.
PG  - 86-91
LID - 10.1159/000215045 [doi]
AB  - The fragment of the membrane protein M gene with high conservation and 
      specificity of porcine reproductive and respiratory syndrome virus (PRRSV) was 
      chosen to be the target region, according to which six special primers were 
      designed successfully. Reverse transcription loop-mediated isothermal 
      amplification (RT-LAMP) was applied to detect the PRRSV by incubation at 65 
      degrees for only 45 min with the ladder-like pattern of bands from 182 bp on the 
      agarose gel, and the product specificity was confirmed by RsaI. The result of 
      RT-LAMP could also be visualized directly with the naked eye by adding the 
      intercalating dye Picogreen. The RT-LAMP was identified to detect only the PRRSV 
      in all viruses tested, which demonstrated the high specificity. By using various 
      sample dilutions as templates, the sensitivity of RT-LAMP was found to be 
      100-fold higher than that of RT-PCR and could be comparable to the fluorescence 
      quantitative RT-PCR. A comparison was obtained by the RT-LAMP and PCR assays 
      using 20 clinical samples. Finally, a rapid, convenient and reliable PRRSV 
      detection system was developed using the RT-LAMP.
CI  - Copyright (c) 2009 S. Karger AG, Basel.
FAU - Qin, Chen
AU  - Qin C
AD  - School of Life Science, Shanghai University, Shanghai, People's Republic of 
      China. chenqincc@yahoo.com.cn
FAU - Jian, Li
AU  - Jian L
FAU - Xue-En, Fang
AU  - Xue-En F
FAU - Wei, Xiong
AU  - Wei X
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20090428
PL  - Switzerland
TA  - Intervirology
JT  - Intervirology
JID - 0364265
RN  - 0 (DNA Primers)
RN  - 0 (M protein, porcine reproductive and respiratory syndrome virus)
RN  - 0 (Organic Chemicals)
RN  - 0 (PicoGreen)
RN  - 0 (Viral Matrix Proteins)
SB  - IM
MH  - Animals
MH  - Blood/virology
MH  - DNA Primers/genetics
MH  - Electrophoresis, Agar Gel
MH  - Lung/virology
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Organic Chemicals/metabolism
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*isolation & 
      purification
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Temperature
MH  - Time Factors
MH  - Viral Matrix Proteins/genetics
EDAT- 2009/04/30 09:00
MHDA- 2009/07/03 09:00
CRDT- 2009/04/30 09:00
PHST- 2008/10/06 00:00 [received]
PHST- 2009/02/18 00:00 [accepted]
PHST- 2009/04/30 09:00 [entrez]
PHST- 2009/04/30 09:00 [pubmed]
PHST- 2009/07/03 09:00 [medline]
AID - 000215045 [pii]
AID - 10.1159/000215045 [doi]
PST - ppublish
SO  - Intervirology. 2009;52(2):86-91. doi: 10.1159/000215045. Epub 2009 Apr 28.