PMID- 19402188
OWN - NLM
STAT- MEDLINE
DCOM- 20090724
LR  - 20211020
IS  - 1099-1565 (Electronic)
IS  - 0958-0344 (Print)
IS  - 0958-0344 (Linking)
VI  - 20
IP  - 4
DP  - 2009 Jul-Aug
TI  - Comparison of the phenolic component profiles of skullcap (Scutellaria 
      lateriflora) and germander (Teucrium canadense and T. chamaedrys), a potentially 
      hepatotoxic adulterant.
PG  - 298-306
LID - 10.1002/pca.1127 [doi]
AB  - INTRODUCTION: Scutellaria lateriflora, commonly known as skullcap, is used as an 
      ingredient in numerous herbal products. Unfortunately, it has occasionally been 
      adulterated with Teucrium canadense or T. chamaedrys, commonly known as 
      germander, which contains potentially hepatotoxic diterpenes. Chromatographic 
      profiles of the phenolic components provide a means of distinguishing between 
      these plants and enhancing public safety. OBJECTIVE: To develop a chromatographic 
      method for the identification of Scutellaria lateriflora and two Teucrium species 
      and to quantify the latter as adulterants. METHODOLOGY: Samples were extracted 
      with aqueous methanol and the extracts were analysed using a standardised 
      LC-DAD-ESI/MS profiling method to obtain their phenolic profiles. RESULTS: 
      Skullcap contained primarily flavonoids, while the major phenolic components of 
      the two Teucrium species were the phenylethanoids, verbascoside and teucrioside. 
      Using the phenylethanoids as markers, it was possible to clearly distinguish 
      between the two genus and to determine 5% Teucrium mixed with Scutellaria using 
      either ultraviolet absorption spectrometry or mass spectrometry in the total ion 
      count mode. Using MS in the selective ion monitoring (SIM) mode, 1% Teucrium 
      could be measured. CONCLUSIONS: This study showed that chromatographic profiling 
      was able to identify Scutellaria and Teucrium, separately and when mixed 
      together.
FAU - Lin, Long-Ze
AU  - Lin LZ
AD  - Human Nutrition Research Center, Agricultural Research Service, US Department of 
      Agriculture, 103000 Baltimore Avenue, Building-161, BARC-East, Beltsville, MD 
      20705, USA. longze.lin@ ars.usda.gov
FAU - Harnly, James M
AU  - Harnly JM
FAU - Upton, Roy
AU  - Upton R
LA  - eng
GR  - Y01 OD001298-01/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Phytochem Anal
JT  - Phytochemical analysis : PCA
JID - 9200492
RN  - 0 (Phenols)
SB  - IM
MH  - Drug Contamination
MH  - Molecular Structure
MH  - Phenols/*chemistry
MH  - Plants, Medicinal/*chemistry
MH  - Scutellaria/*chemistry
MH  - Teucrium/*chemistry
PMC - PMC3583524
MID - NIHMS322961
EDAT- 2009/04/30 09:00
MHDA- 2009/07/25 09:00
PMCR- 2013/02/27
CRDT- 2009/04/30 09:00
PHST- 2009/04/30 09:00 [entrez]
PHST- 2009/04/30 09:00 [pubmed]
PHST- 2009/07/25 09:00 [medline]
PHST- 2013/02/27 00:00 [pmc-release]
AID - 10.1002/pca.1127 [doi]
PST - ppublish
SO  - Phytochem Anal. 2009 Jul-Aug;20(4):298-306. doi: 10.1002/pca.1127.