PMID- 19403161
OWN - NLM
STAT- MEDLINE
DCOM- 20100816
LR  - 20161125
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 112
IP  - 3
DP  - 2010 May
TI  - Bone formation in a rat calvarial defect model after transplanting autogenous 
      bone marrow with beta-tricalcium phosphate.
PG  - 270-7
LID - 10.1016/j.acthis.2009.01.003 [doi]
AB  - In the present study, we evaluated the osteogenic potential of an autogenous bone 
      marrow graft combined with beta-tricalcium phosphate (beta-TCP) in a rat 
      calvarial bone defect model. The bone marrow harvested from the tibia of 
      7-week-old rats was grafted autogenously in a calvarial defect together with 
      beta-TCP (=BTG group, n=16) or without beta-TCP (=BG group, n=16). Groups of 
      animals were also treated with beta-TCP alone (=TG group, n=16) and control 
      animals (n=8) received no graft implanted into the defect. We then observed the 
      process of bone formation by histology, enzyme histochemistry and 
      immunohistochemistry. Five days after grafting, in the BTG and BG groups, cell 
      proliferation and osteogenic differentiation were observed. From 5 to 10 days 
      after surgery, active Runx2, osteopontin (OPN), and TRAP- positive cells appeared 
      in the BTG and BG groups. New bone formation started in the defect in both the 
      BTG and BG groups. At 30 days after grafting, the BTG group showed new bone 
      development and replacement of beta-TCP to fill the bone defect. New bone 
      formation in the BTG group was significantly greater than in the BG group 
      (P<0.01). The TG group showed no marked bone formation in the defect. The 
      combination graft of bone marrow with beta-TCP showed marked bone formation in 
      rat calvarial defects. Our results indicate that the combination grafts of bone 
      marrow with beta-TCP may be an effective technique for repairing bone defects 
      Beta-TCPgraft (TG) group.
CI  - 2009 Elsevier GmbH. All rights reserved.
FAU - Shirasu, Nobuaki
AU  - Shirasu N
AD  - Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University 
      Medical and Dental School, 2-5-1 Shikata, Okayama-city 7008525, Japan.
FAU - Ueno, Takaaki
AU  - Ueno T
FAU - Hirata, Yasuhisa
AU  - Hirata Y
FAU - Hirata, Azumi
AU  - Hirata A
FAU - Kagawa, Toshimasa
AU  - Kagawa T
FAU - Kanou, Miwa
AU  - Kanou M
FAU - Sawaki, Masako
AU  - Sawaki M
FAU - Wakimoto, Mari
AU  - Wakimoto M
FAU - Ota, Akiko
AU  - Ota A
FAU - Imura, Hideto
AU  - Imura H
FAU - Matsumura, Tatsushi
AU  - Matsumura T
FAU - Yamada, Tomohiro
AU  - Yamada T
FAU - Yamachika, Eiki
AU  - Yamachika E
FAU - Sano, Kazuo
AU  - Sano K
LA  - eng
PT  - Journal Article
DEP - 20090429
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (Biocompatible Materials)
RN  - 0 (Bone Substitutes)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Isoenzymes)
RN  - 0 (beta-tricalcium phosphate)
RN  - 106441-73-0 (Osteopontin)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
SB  - IM
MH  - Acid Phosphatase/metabolism
MH  - Animals
MH  - Biocompatible Materials/*pharmacology
MH  - Bone Marrow Cells/cytology/drug effects/*metabolism
MH  - *Bone Marrow Transplantation
MH  - *Bone Regeneration
MH  - Bone Substitutes/pharmacology
MH  - Calcium Phosphates/*pharmacology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Core Binding Factor Alpha 1 Subunit/metabolism
MH  - Disease Models, Animal
MH  - Isoenzymes/metabolism
MH  - Male
MH  - *Osteogenesis/drug effects/physiology
MH  - Osteopontin/metabolism
MH  - Rats
MH  - Skull/injuries/pathology
MH  - Tartrate-Resistant Acid Phosphatase
MH  - Tissue Engineering
MH  - Wound Healing/drug effects/physiology
EDAT- 2009/05/01 09:00
MHDA- 2010/08/17 06:00
CRDT- 2009/05/01 09:00
PHST- 2008/08/17 00:00 [received]
PHST- 2009/01/13 00:00 [revised]
PHST- 2009/01/26 00:00 [accepted]
PHST- 2009/05/01 09:00 [entrez]
PHST- 2009/05/01 09:00 [pubmed]
PHST- 2010/08/17 06:00 [medline]
AID - S0065-1281(09)00005-1 [pii]
AID - 10.1016/j.acthis.2009.01.003 [doi]
PST - ppublish
SO  - Acta Histochem. 2010 May;112(3):270-7. doi: 10.1016/j.acthis.2009.01.003. Epub 
      2009 Apr 29.