PMID- 19403409
OWN - NLM
STAT- MEDLINE
DCOM- 20100111
LR  - 20090430
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 29
IP  - 4
DP  - 2009 Apr
TI  - [Alterations of peripheral blood endothelial progenitor cells from patients with 
      ST elevation myocardial infarction after snake venom fibrinolytic enzyme 
      therapy].
PG  - 734-7
AB  - OBJECTIVE: To investigate the morphological and quantitative alterations of 
      peripheral blood endothelial progenitor cells (EPCs) in patients with ST 
      elevation myocardial infarction (STEMI) after snake venom fibrinolytic enzyme 
      therapy. METHODS: Sixty patients with STEMI and 20 control patients free of STEMI 
      were enrolled in this study. The mononuclear cells were isolated from the 
      peripheral blood of the STEMI patients before and 3 and 7 days after fibrinolytic 
      enzyme injection. The isolated cells were cultured in RPMI1640 medium 
      supplemented with vascular endothelial growth factor 165 (VEGF165) and basic 
      fibroblast growth factor (bFGF). The EPCs were characterized as adherent cells of 
      positive for both DiL-acLDL and FITC-UEA-I by direct fluorescent staining. The 
      cell morphology was observed and the number of the colony-forming units (CFU) was 
      determined by phase-contrast microscope. RESULTS: The number of the EPCs 
      increased obviously in patients with STEMI 3 days after fibrinolytic enzyme 
      injection, and reduced 7 days after the injection but remained greater than that 
      in the control group. The injection of fibrinolytic enzyme did not result obvious 
      changes in the number of CFU in the patients with STEMI. CONCLUSION: Snake venom 
      fibrinolytic enzyme injection can increase the peripheral blood EPCs in patients 
      with STEMI.
FAU - Jiang, Chao-xin
AU  - Jiang CX
AD  - Clinical Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 
      510282, China. jiangchaoxin@163.com
FAU - Wang, Qian
AU  - Wang Q
FAU - Zheng, Lei
AU  - Zheng L
FAU - Bao, Jie
AU  - Bao J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Snake Venoms)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Count
MH  - Electrocardiography
MH  - Endothelial Cells/*cytology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Myocardial Infarction/*diagnosis/*drug therapy/pathology
MH  - *Snake Venoms
MH  - Stem Cells/*drug effects/pathology
MH  - *Thrombolytic Therapy
EDAT- 2009/05/01 09:00
MHDA- 2010/01/12 06:00
CRDT- 2009/05/01 09:00
PHST- 2009/05/01 09:00 [entrez]
PHST- 2009/05/01 09:00 [pubmed]
PHST- 2010/01/12 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2009 Apr;29(4):734-7.