PMID- 19404216
OWN - NLM
STAT- MEDLINE
DCOM- 20090730
LR  - 20181201
IS  - 1556-1380 (Electronic)
IS  - 1556-0864 (Linking)
VI  - 4
IP  - 6
DP  - 2009 Jun
TI  - Epidermal growth factor receptor gene in primary tumor and metastatic sites from 
      non-small cell lung cancer.
PG  - 684-8
LID - 10.1097/JTO.0b013e3181a52359 [doi]
AB  - INTRODUCTION: The majority of patients with non-small cell lung cancer (NSCLC) 
      develop distant metastases. Epidermal growth factor receptor (EGFR) tyrosine 
      kinase inhibitors are capable of reducing brain and adrenal metastases. However, 
      the EGFR status may be discordant between primary NSCLC and the corresponding 
      metastases. METHODS: Using fluorescence in situ hybridization (FISH) analysis, 
      the EGFR gene status was evaluated in a series of 38 cerebral or adrenal 
      metastases collected from two institutions and in the corresponding primary 
      tumors. Also, EGFR mutational analysis was performed using direct sequencing on 
      the cerebral metastases. RESULTS: EGFR FISH was positive in 28% of the primary 
      tumors and in 45% of the metastases (p < 0.05). Among the seven cases 
      FISH-positive at the metastatic site but negative in the primary tumor, six were 
      brain metastases, and one was an adrenal metastasis; all were polysomic for 
      chromosome 7, none were amplified. No EGFR mutations have been found in the 
      cerebral metastases. CONCLUSION: Because the molecular asset of EGFR may change 
      during the metastatic progression of NSCLC to brain (but not to adrenal), the 
      selection of patients with brain metastasis for specific targeted therapies by 
      EGFR FISH analysis should be performed on metastatic lesions rather than on their 
      corresponding primary tumors.
FAU - Daniele, Lorenzo
AU  - Daniele L
AD  - Department of Biomedical Sciences and Human Oncology, University of Turin, Turin, 
      Italy. lorenzo.daniele@unito.it
FAU - Cassoni, Paola
AU  - Cassoni P
FAU - Bacillo, Elisa
AU  - Bacillo E
FAU - Cappia, Susanna
AU  - Cappia S
FAU - Righi, Luisella
AU  - Righi L
FAU - Volante, Marco
AU  - Volante M
FAU - Tondat, Fabrizio
AU  - Tondat F
FAU - Inghirami, Giorgio
AU  - Inghirami G
FAU - Sapino, Anna
AU  - Sapino A
FAU - Scagliotti, Giorgio V
AU  - Scagliotti GV
FAU - Papotti, Mauro
AU  - Papotti M
FAU - Novello, Silvia
AU  - Novello S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Thorac Oncol
JT  - Journal of thoracic oncology : official publication of the International 
      Association for the Study of Lung Cancer
JID - 101274235
RN  - 0 (DNA, Neoplasm)
RN  - EC 2.7.10.1 (EGFR protein, human)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Adenocarcinoma/genetics/secondary
MH  - Adrenal Gland Neoplasms/*genetics/secondary
MH  - Aged
MH  - Aged, 80 and over
MH  - Brain Neoplasms/*genetics/secondary
MH  - Carcinoma, Large Cell/genetics/secondary
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/secondary
MH  - Carcinoma, Squamous Cell/genetics/secondary
MH  - Chromosomes, Human, Pair 7/genetics
MH  - DNA, Neoplasm/genetics/metabolism
MH  - ErbB Receptors/*genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Small Cell Lung Carcinoma/genetics/secondary
MH  - Survival Rate
EDAT- 2009/05/01 09:00
MHDA- 2009/07/31 09:00
CRDT- 2009/05/01 09:00
PHST- 2009/05/01 09:00 [entrez]
PHST- 2009/05/01 09:00 [pubmed]
PHST- 2009/07/31 09:00 [medline]
AID - S1556-0864(15)32506-5 [pii]
AID - 10.1097/JTO.0b013e3181a52359 [doi]
PST - ppublish
SO  - J Thorac Oncol. 2009 Jun;4(6):684-8. doi: 10.1097/JTO.0b013e3181a52359.