PMID- 19407393
OWN - NLM
STAT- MEDLINE
DCOM- 20090619
LR  - 20221207
IS  - 1744-3091 (Electronic)
IS  - 1744-3091 (Linking)
VI  - 65
IP  - Pt 5
DP  - 2009 May 1
TI  - Purification, crystallization and preliminary X-ray diffraction analysis of a 
      plant subtilase.
PG  - 522-5
LID - 10.1107/S1744309109013979 [doi]
AB  - The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato 
      cell culture and crystallized using the sitting-drop vapour-diffusion method. A 
      native data set was collected to 2.5 A resolution at 100 K using synchrotron 
      radiation. For experimental phasing, CsCl-derivative and 
      tetrakis(acetoxymercuri)methane (TAMM) derivative crystals were employed for 
      MIRAS phasing. Three caesium sites and one TAMM site were identified, which 
      allowed solution of the structure.
FAU - Rose, Rolf
AU  - Rose R
AD  - Chemical Genomics Centre, Dortmund, Germany.
FAU - Huttenlocher, Franziska
AU  - Huttenlocher F
FAU - Cedzich, Anna
AU  - Cedzich A
FAU - Kaiser, Markus
AU  - Kaiser M
FAU - Schaller, Andreas
AU  - Schaller A
FAU - Ottmann, Christian
AU  - Ottmann C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090428
PL  - England
TA  - Acta Crystallogr Sect F Struct Biol Cryst Commun
JT  - Acta crystallographica. Section F, Structural biology and crystallization 
      communications
JID - 101226117
RN  - EC 3.4.21.- (Subtilisins)
RN  - EC 3.4.21.- (subtilase, plant)
SB  - IM
MH  - Crystallization
MH  - Crystallography, X-Ray
MH  - Solanum lycopersicum/*enzymology/genetics
MH  - Subtilisins/*analysis/*chemistry/isolation & purification/metabolism
PMC - PMC2675601
EDAT- 2009/05/02 09:00
MHDA- 2009/06/20 09:00
PMCR- 2011/05/01
CRDT- 2009/05/02 09:00
PHST- 2009/03/17 00:00 [received]
PHST- 2009/04/14 00:00 [accepted]
PHST- 2009/05/02 09:00 [entrez]
PHST- 2009/05/02 09:00 [pubmed]
PHST- 2009/06/20 09:00 [medline]
PHST- 2011/05/01 00:00 [pmc-release]
AID - S1744309109013979 [pii]
AID - bw5291 [pii]
AID - 10.1107/S1744309109013979 [doi]
PST - ppublish
SO  - Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 May 1;65(Pt 5):522-5. doi: 
      10.1107/S1744309109013979. Epub 2009 Apr 28.