PMID- 19409874
OWN - NLM
STAT- MEDLINE
DCOM- 20090609
LR  - 20211020
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 384
IP  - 2
DP  - 2009 Jun 26
TI  - The angiotensin II-AT1 receptor stimulates reactive oxygen species within the 
      cell nucleus.
PG  - 149-54
LID - 10.1016/j.bbrc.2009.04.126 [doi]
AB  - We and others have reported significant expression of the Ang II Type 1 receptor 
      (AT1R) on renal nuclei; thus, the present study assessed the functional pathways 
      and distribution of the intracellular AT1R on isolated nuclei. Ang II (1nM) 
      stimulated DCF fluorescence, an intranuclear indicator of reactive oxygen species 
      (ROS), while the AT1R antagonist losartan or the NADPH oxidase (NOX) inhibitor 
      DPI abolished the increase in ROS. Dual labeling of nuclei with antibodies 
      against nucleoporin 62 (Nup62) and AT1R or the NADPH oxidase isoform NOX4 
      revealed complete overlap of the Nup62 and AT1R (99%) by flow cytometry, while 
      NOX4 was present on 65% of nuclei. Treatment of nuclei with a PKC agonist 
      increased ROS while the PKC inhibitor GF109203X or PI3 kinase inhibitor LY294002 
      abolished Ang II stimulation of ROS. We conclude that the Ang II-AT1R-PKC axis 
      may directly influence nuclear function within the kidney through a redox 
      sensitive pathway.
FAU - Pendergrass, Karl D
AU  - Pendergrass KD
AD  - The Hypertension and Vascular Research Center, Department of Physiology and 
      Pharmacology, Wake Forest University School of Medicine, Medical Center 
      Boulevard, Winston-Salem, NC 27157, USA.
FAU - Gwathmey, Tanya M
AU  - Gwathmey TM
FAU - Michalek, Ryan D
AU  - Michalek RD
FAU - Grayson, Jason M
AU  - Grayson JM
FAU - Chappell, Mark C
AU  - Chappell MC
LA  - eng
GR  - P01 HL051952/HL/NHLBI NIH HHS/United States
GR  - HD-47584/HD/NICHD NIH HHS/United States
GR  - R01 HL056973/HL/NHLBI NIH HHS/United States
GR  - R01 HL056973-09/HL/NHLBI NIH HHS/United States
GR  - HL-56973/HL/NHLBI NIH HHS/United States
GR  - P01 HL051952-150007/HL/NHLBI NIH HHS/United States
GR  - HD-17644/HD/NICHD NIH HHS/United States
GR  - R29 HL056973/HL/NHLBI NIH HHS/United States
GR  - HL-51952/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090503
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Angiotensin II Type 1 Receptor Blockers)
RN  - 0 (Indoles)
RN  - 0 (Maleimides)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptor, Angiotensin, Type 1)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - JMS50MPO89 (Losartan)
RN  - L79H6N0V6C (bisindolylmaleimide I)
SB  - IM
MH  - Angiotensin II Type 1 Receptor Blockers/pharmacology
MH  - Animals
MH  - Cell Nucleus/*metabolism
MH  - Indoles/pharmacology
MH  - Kidney/cytology/*metabolism
MH  - Losartan/pharmacology
MH  - Male
MH  - Maleimides/pharmacology
MH  - Protein Kinase C/antagonists & inhibitors/metabolism
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Reactive Oxygen Species/*metabolism
MH  - Receptor, Angiotensin, Type 1/*metabolism
PMC - PMC2688692
MID - NIHMS114065
EDAT- 2009/05/05 09:00
MHDA- 2009/06/10 09:00
PMCR- 2010/06/26
CRDT- 2009/05/05 09:00
PHST- 2009/03/13 00:00 [received]
PHST- 2009/04/15 00:00 [accepted]
PHST- 2009/05/05 09:00 [entrez]
PHST- 2009/05/05 09:00 [pubmed]
PHST- 2009/06/10 09:00 [medline]
PHST- 2010/06/26 00:00 [pmc-release]
AID - S0006-291X(09)00778-5 [pii]
AID - 10.1016/j.bbrc.2009.04.126 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2009 Jun 26;384(2):149-54. doi: 
      10.1016/j.bbrc.2009.04.126. Epub 2009 May 3.