PMID- 19412570
OWN - NLM
STAT- MEDLINE
DCOM- 20091209
LR  - 20151119
IS  - 1436-0691 (Electronic)
IS  - 0944-1166 (Linking)
VI  - 16
IP  - 5
DP  - 2009
TI  - Increased K-ras mutation and expression of S100A4 and MUC2 protein in the 
      malignant intraductal papillary mucinous tumor of the pancreas.
PG  - 668-74
LID - 10.1007/s00534-009-0105-7 [doi]
AB  - PURPOSE: The purpose of this study was to document the biological changes during 
      the progression of intraductal papillary mucinous neoplasm of the pancreas (IPMN) 
      and to identify biological markers capable of differentiating benign and 
      malignant IPMN. METHODS: Forty-one patients with IPMN who underwent resection 
      between 1994 and 2003 were enrolled in this study. The paraffin-embedded tumors 
      from 27 with benign IPMNs and from 14 with IPMCs were subjected to 
      immunohistochemical staining and DNA extraction. Direct DNA sequencing analysis 
      for K-ras mutation and immunohistochemical staining using 17 biological markers 
      was performed. RESULTS: K-ras mutations at codon 12 and 13 were detected in 13 of 
      37 (38.2%) of the IPMNs: in 5 of 24 (20.8%) of benign IPMNs, and in 8 of 13 
      (61.5%) of malignant IPMNs (p = 0.028). The expression of S100A4 and MUC2 were 
      increased in malignant IPMNs. S100A4 was expressed in 2 (7.4%) of 27 benign 
      IPMNs, and 6 (42.9%) of 14 malignant IPMNs (p = 0.007). MUC2 was expressed in 2 
      (7.4%) benign IPMNs, and in 9 (64.3%) malignant IPMNs (p < 0.001). CONCLUSION: 
      K-ras mutation and the expression of S100A4 and MUC2 (especially in intestinal 
      subtype) were found to be related to malignancy in IPMN, and may be useful for 
      the diagnosis and for assessing the biological behavior of IPMN.
FAU - Jang, Jin-Young
AU  - Jang JY
AD  - Department of Surgery, Seoul National University College of Medicine, 28 
      Yongon-dong, Chongno-gu, Seoul, 110-744, South Korea.
FAU - Park, Yoon-Chan
AU  - Park YC
FAU - Song, Yoon Sup
AU  - Song YS
FAU - Lee, Seung Eun
AU  - Lee SE
FAU - Hwang, Dae Wook
AU  - Hwang DW
FAU - Lim, Chang-Sup
AU  - Lim CS
FAU - Lee, Hee Eun
AU  - Lee HE
FAU - Kim, Woo Ho
AU  - Kim WH
FAU - Kim, Sun-Whe
AU  - Kim SW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090502
PL  - Japan
TA  - J Hepatobiliary Pancreat Surg
JT  - Journal of hepato-biliary-pancreatic surgery
JID - 9431940
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Mucin-2)
RN  - 0 (S100 Proteins)
SB  - IM
MH  - Adenocarcinoma, Mucinous/genetics/mortality/pathology
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/analysis/*genetics
MH  - Biopsy, Needle
MH  - Carcinoma, Pancreatic Ductal/*genetics/mortality/pathology
MH  - Cohort Studies
MH  - Disease Progression
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Genes, ras
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Mucin-2/*genetics/metabolism
MH  - Mutation
MH  - Neoplasm Staging
MH  - Pancreatic Neoplasms/*genetics/mortality/pathology
MH  - Polymerase Chain Reaction
MH  - Probability
MH  - Prognosis
MH  - Retrospective Studies
MH  - S100 Proteins/genetics/*metabolism
MH  - Statistics, Nonparametric
MH  - Survival Rate
EDAT- 2009/05/05 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/05/05 09:00
PHST- 2008/09/10 00:00 [received]
PHST- 2009/02/01 00:00 [accepted]
PHST- 2009/05/05 09:00 [entrez]
PHST- 2009/05/05 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - 10.1007/s00534-009-0105-7 [doi]
PST - ppublish
SO  - J Hepatobiliary Pancreat Surg. 2009;16(5):668-74. doi: 10.1007/s00534-009-0105-7. 
      Epub 2009 May 2.