PMID- 19415548
OWN - NLM
STAT- MEDLINE
DCOM- 20090722
LR  - 20090505
IS  - 1521-0499 (Electronic)
IS  - 0190-2148 (Linking)
VI  - 35
IP  - 4
DP  - 2009 May
TI  - Lipopolysaccharide stimulation of dendritic cells induces interleukin-10 
      producing allergen-specific T cells in vitro but fails to prevent allergic airway 
      disease.
PG  - 307-23
LID - 10.1080/01902140802709460 [doi]
AB  - Dendritic cells (DCs) play an important role in directing naive T cells towards a 
      Th1/Th2 or regulatory T cells (Treg) cell phenotype. In this context, interleukin 
      (IL)-10 has been shown to exhibit immune regulatory capacities. The aim of this 
      study was to delineate the influence of high-IL-10-producing DCs on DC-T-cell 
      interactions in inhibiting allergen-induced airway inflammation and 
      hyperreactivity in a murine model of allergic airway disease. Bone marrow-derived 
      dendritic cells (BMDCs) were generated from hemopoietic progenitors by culture 
      with granulocyte-macrophage colony-stimulating factor (GM-CSF), and stimulated 
      with ovalbumin (OVA) +/- lipopolysaccharide (LPS). The effects of 
      ovalbumin-pulsed BMDCs on cytokine production by allergen-specific naive T cells 
      were studied in vitro. The development of airway inflammation in Balb/c mice was 
      determined after intranasal administration of BMDCs in vivo. LPS stimulation of 
      BMDCs strongly enhanced IL-10 production. Coculture of LPS-modulated DCs 
      exhibiting increased IL-10 production with allergen-specific naive T cells 
      reduced the production of interferon (IFN)-gamma and IL-5, but enhanced the 
      production of IL-10. After blockade with anti-IL-10 plus anti-IL-10-receptor 
      antibodies, the level of IFN-gamma and IL-5 production by cocultured T cells was 
      restored, underlining the regulatory function of IL-10. Intranasal administration 
      of high-IL-10-producing LPS-stimulated, OVA-primed BMDCs prior to repetitive 
      airway allergen challenges resulted in an even enhanced airway inflammation. 
      These data demonstrate that increased IL-10 production by DCs may be a critical 
      element for T-cell activation and differentiation in the context of 
      allergen-induced immune responses in vitro. However, this DC modulation did not 
      translate into suppression of allergic airway disease in vivo.
FAU - Ahrens, Birgit
AU  - Ahrens B
AD  - Department of Pediatric Pneumology and Immunology, Charite, University Medicine 
      Berlin, Germany. birgit.ahrens@charite.de
FAU - Freund, Tobias
AU  - Freund T
FAU - Rha, Ro-Dug
AU  - Rha RD
FAU - Dittrich, Anna-Maria
AU  - Dittrich AM
FAU - Quarcoo, David
AU  - Quarcoo D
FAU - Hutloff, Andreas
AU  - Hutloff A
FAU - Hamelmann, Eckard
AU  - Hamelmann E
LA  - eng
PT  - Journal Article
PL  - England
TA  - Exp Lung Res
JT  - Experimental lung research
JID - 8004944
RN  - 0 (Allergens)
RN  - 0 (Interleukin-5)
RN  - 0 (Lipopolysaccharides)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Asthma/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/drug effects/*immunology
MH  - Female
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-10/*biosynthesis
MH  - Interleukin-5/biosynthesis
MH  - Lipopolysaccharides/pharmacology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/immunology
MH  - T-Lymphocytes/*immunology
EDAT- 2009/05/06 09:00
MHDA- 2009/07/23 09:00
CRDT- 2009/05/06 09:00
PHST- 2009/05/06 09:00 [entrez]
PHST- 2009/05/06 09:00 [pubmed]
PHST- 2009/07/23 09:00 [medline]
AID - 910943457 [pii]
AID - 10.1080/01902140802709460 [doi]
PST - ppublish
SO  - Exp Lung Res. 2009 May;35(4):307-23. doi: 10.1080/01902140802709460.