PMID- 19416349
OWN - NLM
STAT- MEDLINE
DCOM- 20090625
LR  - 20211203
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 68
IP  - 3
DP  - 2009 Jun
TI  - Molecular detection of uncultured cyanobacteria and aminotransferase domains for 
      cyanotoxin production in sediments of different Kenyan lakes.
PG  - 340-50
LID - 10.1111/j.1574-6941.2009.00678.x [doi]
AB  - PCR-based denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA 
      gene fragments was used to identify the cyanobacterial phylotypes in sediments 
      and plankton of saline-alkaline and freshwater lakes of Kenya. The detection of 
      the aminotransferase domain located on modules mcyE and ndaF using specific 
      molecular markers confirmed the presence of potential toxin-producing 
      cyanobacteria. The eight nucleotide sequences obtained from DGGE bands were 
      placed in three divergent cyanobacterial clusters. Five nucleotide sequences were 
      close to members of the genera Anabaenopsis and Umezakia (Nostocales), two 
      sequences fell in the cluster with Arthrospira sp. (Oscillatoriales) and one 
      sequence was related to Chroococcidiopsis sp. (Pleurocapsales). The presence of 
      the latter taxon was demonstrated de novo in the investigated lakes. All nine 
      attained nucleotide sequences of the aminotransferase region belonged to the mcyE 
      module. Five sequences of the aminotransferase domain were included in the 
      cluster having the nucleotide sequence of Anabaena sp. but showed a separate 
      lineage. Other four aminotransferases were placed in the cluster represented by 
      nucleotide sequence of Microcystis aeruginosa. To our knowledge, this is the 
      first report on molecular detection of cyanobacterial phylotypes in sediments of 
      African lakes and aminotransferase domains for cyanotoxin production from 
      sediment samples in general.
FAU - Dadheech, Pawan K
AU  - Dadheech PK
AD  - Department of Limnology of Stratified Lakes, Leibniz-Institute of Freshwater 
      Ecology and Inland Fisheries, Stechlin, Germany.
FAU - Krienitz, Lothar
AU  - Krienitz L
FAU - Kotut, Kiplagat
AU  - Kotut K
FAU - Ballot, Andreas
AU  - Ballot A
FAU - Casper, Peter
AU  - Casper P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090414
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Bacterial Toxins)
RN  - 0 (Cyanobacteria Toxins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Marine Toxins)
RN  - 0 (Microcystins)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 2.6.1.- (Transaminases)
SB  - IM
MH  - Bacterial Toxins/*genetics
MH  - Cyanobacteria/classification/*genetics/isolation & purification
MH  - Cyanobacteria Toxins
MH  - DNA, Bacterial/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Fresh Water/microbiology
MH  - Geologic Sediments/*microbiology
MH  - Kenya
MH  - Marine Toxins/*genetics
MH  - Microcystins/*genetics
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
MH  - Transaminases/genetics
MH  - *Water Microbiology
EDAT- 2009/05/07 09:00
MHDA- 2009/06/26 09:00
CRDT- 2009/05/07 09:00
PHST- 2009/05/07 09:00 [entrez]
PHST- 2009/05/07 09:00 [pubmed]
PHST- 2009/06/26 09:00 [medline]
AID - FEM678 [pii]
AID - 10.1111/j.1574-6941.2009.00678.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2009 Jun;68(3):340-50. doi: 
      10.1111/j.1574-6941.2009.00678.x. Epub 2009 Apr 14.