PMID- 19417738
OWN - NLM
STAT- MEDLINE
DCOM- 20091013
LR  - 20211020
IS  - 1525-0024 (Electronic)
IS  - 1525-0016 (Print)
IS  - 1525-0016 (Linking)
VI  - 17
IP  - 7
DP  - 2009 Jul
TI  - Engraftment of bone marrow-derived stem cells to the lung in a model of acute 
      respiratory infection by Pseudomonas aeruginosa.
PG  - 1257-65
LID - 10.1038/mt.2009.96 [doi]
AB  - Stem cell therapy presents an attractive approach to cure cystic fibrosis (CF) 
      lung disease. We set out to investigate the effect of epithelial damage caused by 
      Pseudomonas aeruginosa, a pathogenic bacterium widely occurring in CF, on the 
      engraftment of bone marrow cells in airway epithelium. Intravenous or 
      intratracheal administration of unfractionated green fluorescent protein (GFP(+)) 
      bone marrow cells in P. aeruginosa-infected mice resulted in none or very few 
      GFP(+) cells detected in the lungs of the recipient mice, respectively. Only when 
      GFP(+) bone marrow cells were purified to obtain a cell suspension enriched in 
      progenitor cells and injected intratracheally, significant numbers of GFP(+) 
      cells were detected. Localization of the donor cells at the level of airway 
      epithelium was confirmed by Y-chromosome fluorescence in situ hybridization 
      (FISH) analysis. All donor-derived Y-chromosome(+) cells were found to express 
      cytokeratin (CK). The fractions of GFP(+) cells expressing CK were 0.34 and 0.76% 
      for the 10(5) and 10(6) colony forming units (cfu) bacterial inoculums, 
      respectively. When scored by Y-chromosome positivity these numbers were 0.60 and 
      1.12%, respectively. Our results show for the first time that tissue damage 
      inflicted by bacteria like P. aeruginosa facilitates the airway engraftment of 
      heterologous bone marrow-derived stem cells and their epithelial transformation.
FAU - Rejman, Joanna
AU  - Rejman J
AD  - Institute for the Experimental Treatment of Cystic Fibrosis, H.S. Raffaele 
      Scientific Institute, Milan, Italy.
FAU - Colombo, Carla
AU  - Colombo C
FAU - Conese, Massimo
AU  - Conese M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090505
PL  - United States
TA  - Mol Ther
JT  - Molecular therapy : the journal of the American Society of Gene Therapy
JID - 100890581
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/*physiology
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Cell- and Tissue-Based Therapy/*methods
MH  - Lung/metabolism/pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pseudomonas Infections/*therapy
MH  - Pseudomonas aeruginosa/physiology
MH  - Respiratory Tract Infections/microbiology/*therapy
MH  - Stem Cells/cytology/physiology
PMC - PMC2835203
EDAT- 2009/05/07 09:00
MHDA- 2009/10/14 06:00
PMCR- 2010/07/01
CRDT- 2009/05/07 09:00
PHST- 2009/05/07 09:00 [entrez]
PHST- 2009/05/07 09:00 [pubmed]
PHST- 2009/10/14 06:00 [medline]
PHST- 2010/07/01 00:00 [pmc-release]
AID - S1525-0016(16)31837-8 [pii]
AID - 10.1038/mt.2009.96 [doi]
PST - ppublish
SO  - Mol Ther. 2009 Jul;17(7):1257-65. doi: 10.1038/mt.2009.96. Epub 2009 May 5.