PMID- 19417908
OWN - NLM
STAT- MEDLINE
DCOM- 20090828
LR  - 20090506
IS  - 1473-0197 (Print)
IS  - 1473-0189 (Linking)
VI  - 9
IP  - 10
DP  - 2009 May 21
TI  - Microfluidic chips for the crystallization of biomacromolecules by 
      counter-diffusion and on-chip crystal X-ray analysis.
PG  - 1412-21
LID - 10.1039/b819362b [doi]
AB  - Microfluidic devices were designed to perform on micromoles of biological 
      macromolecules and viruses the search and the optimization of crystallization 
      conditions by counter-diffusion, as well as the on-chip analysis of crystals by 
      X-ray diffraction. Chips composed of microchannels were fabricated in 
      poly-dimethylsiloxane (PDMS), poly-methyl-methacrylate (PMMA) and 
      cyclo-olefin-copolymer (COC) by three distinct methods, namely replica casting, 
      laser ablation and hot embossing. The geometry of the channels was chosen to 
      ensure that crystallization occurs in a convection-free environment. The 
      transparency of the materials is compatible with crystal growth monitoring by 
      optical microscopy. The quality of the protein 3D structures derived from on-chip 
      crystal analysis by X-ray diffraction using a synchrotron radiation was used to 
      identify the most appropriate polymers. Altogether the results demonstrate that 
      for a novel biomolecule, all steps from the initial search of crystallization 
      conditions to X-ray diffraction data collection for 3D structure determination 
      can be performed in a single chip.
FAU - Dhouib, Kaouthar
AU  - Dhouib K
AD  - Architecture et reactivite de l'ARN, Universite de Strasbourg, CNRS, IBMC, 15 rue 
      Rene Descartes, F-67084, Strasbourg, France.
FAU - Khan Malek, Chantal
AU  - Khan Malek C
FAU - Pfleging, Wilhelm
AU  - Pfleging W
FAU - Gauthier-Manuel, Bernard
AU  - Gauthier-Manuel B
FAU - Duffait, Roland
AU  - Duffait R
FAU - Thuillier, Gael
AU  - Thuillier G
FAU - Ferrigno, Rosaria
AU  - Ferrigno R
FAU - Jacquamet, Lilian
AU  - Jacquamet L
FAU - Ohana, Jeremy
AU  - Ohana J
FAU - Ferrer, Jean-Luc
AU  - Ferrer JL
FAU - Theobald-Dietrich, Anne
AU  - Theobald-Dietrich A
FAU - Giege, Richard
AU  - Giege R
FAU - Lorber, Bernard
AU  - Lorber B
FAU - Sauter, Claude
AU  - Sauter C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090302
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Macromolecular Substances)
RN  - 63148-62-9 (baysilon)
RN  - 9011-14-7 (Polymethyl Methacrylate)
SB  - IM
MH  - Crystallization
MH  - Crystallography, X-Ray/*instrumentation
MH  - Dimethylpolysiloxanes/chemistry
MH  - Macromolecular Substances/*chemistry
MH  - Microfluidic Analytical Techniques/*instrumentation
MH  - Polymethyl Methacrylate/chemistry
EDAT- 2009/05/07 09:00
MHDA- 2009/08/29 09:00
CRDT- 2009/05/07 09:00
PHST- 2009/05/07 09:00 [entrez]
PHST- 2009/05/07 09:00 [pubmed]
PHST- 2009/08/29 09:00 [medline]
AID - 10.1039/b819362b [doi]
PST - ppublish
SO  - Lab Chip. 2009 May 21;9(10):1412-21. doi: 10.1039/b819362b. Epub 2009 Mar 2.