PMID- 19419898
OWN - NLM
STAT- MEDLINE
DCOM- 20100118
LR  - 20220331
IS  - 1873-4162 (Electronic)
IS  - 1344-6223 (Linking)
VI  - 11
IP  - 4
DP  - 2009 Jul
TI  - Wound age estimation by simultaneous detection of 9 cytokines in human dermal 
      wounds with a multiplex bead-based immunoassay: an estimative method using 
      outsourced examinations.
PG  - 186-90
LID - 10.1016/j.legalmed.2009.03.010 [doi]
AB  - Wound age estimation for human dermal wounds was performed based on 
      quantification of interleukin 1beta (IL 1beta), IL 5, IL 7, IL 12 p70, IL 13, IL 
      17, granulocyte colony-stimulating factor (G-CSF), monocyte chemoattractant 
      protein 1 (MCP 1), and macrophage inflammatory protein 1beta (MIP 1beta). IL 5, 
      IL 12 p 70, IL 13, and IL 17 increased from the early phase, MCP 1 exclusively in 
      the middle phase, and IL 1beta, G-CSF, and MIP 1beta from the middle phase to the 
      late phase. IL 7 decreased from the early phase. Among the cytokines analyzed in 
      the present study, MCP 1 was the most plentiful cytokine. In addition, an 
      outsourced examination, which could be available to any forensic institute, was 
      performed in two cases for confirmative purposes. Many factors have been proposed 
      as markers for dermal wound age estimation, but the set of cytokines selected for 
      the outsourced examination in the present study wound be useful in daily forensic 
      practice.
FAU - Takamiya, Masataka
AU  - Takamiya M
AD  - Department of Legal Medicine, Iwate Medical University School of Medicine, 19-1 
      Uchimaru, Morioka, Iwate 020-8505, Japan. mtakamiy@iwate-med.ac.jp
FAU - Biwasaka, Hitoshi
AU  - Biwasaka H
FAU - Saigusa, Kiyoshi
AU  - Saigusa K
FAU - Nakayashiki, Nori
AU  - Nakayashiki N
FAU - Aoki, Yasuhiro
AU  - Aoki Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090505
PL  - Ireland
TA  - Leg Med (Tokyo)
JT  - Legal medicine (Tokyo, Japan)
JID - 100889186
RN  - 0 (Cytokines)
SB  - IM
MH  - Adult
MH  - Aged, 80 and over
MH  - Cell Proliferation
MH  - Cytokines/*metabolism
MH  - Fibroblasts/metabolism
MH  - Forensic Pathology
MH  - Humans
MH  - Immunoassay/*methods
MH  - Macrophages/metabolism
MH  - Male
MH  - Microspheres
MH  - Neutrophils/metabolism
MH  - Skin/*injuries/*metabolism
MH  - Time Factors
MH  - Wound Healing
EDAT- 2009/05/08 09:00
MHDA- 2010/01/19 06:00
CRDT- 2009/05/08 09:00
PHST- 2009/01/20 00:00 [received]
PHST- 2009/03/14 00:00 [revised]
PHST- 2009/03/17 00:00 [accepted]
PHST- 2009/05/08 09:00 [entrez]
PHST- 2009/05/08 09:00 [pubmed]
PHST- 2010/01/19 06:00 [medline]
AID - S1344-6223(09)00243-0 [pii]
AID - 10.1016/j.legalmed.2009.03.010 [doi]
PST - ppublish
SO  - Leg Med (Tokyo). 2009 Jul;11(4):186-90. doi: 10.1016/j.legalmed.2009.03.010. Epub 
      2009 May 5.