PMID- 19420879
OWN - NLM
STAT- MEDLINE
DCOM- 20090518
LR  - 20191111
IS  - 0040-8891 (Print)
IS  - 0040-8891 (Linking)
VI  - 49
IP  - 4
DP  - 2008 Nov
TI  - Effects of soft-diet feeding on BDNF expression in hippocampus of mice.
PG  - 185-90
AB  - Our previous study showed that mice fed a soft diet after weaning had reduced 
      synaptic connections in the hippocampal formation and impaired spatial learning 
      ability after 3 months of age. We hypothesized that soft-diet feeding during 
      development reduced levels of brain-derived neurotrophic factor (BDNF) protein in 
      the hippocampus, resulting in lower synaptic densities in this region. Male pups 
      of C57BL/6 mice were fed either a solid (hard-diet group) or powdered diet 
      (soft-diet group), starting at weaning. Expression of BDNF protein in the 
      hippocampus and cerebral cortex was evaluated quantitatively with enzyme-linked 
      immunosorbent assay (ELISA) at 1, 3 and 6 months of age. Reduction in BDNF 
      protein levels due to soft diet was detected markedly in the hippocampus of 3- 
      and 6-month-old mice. On the other hand, a soft diet showed no significant effect 
      on BDNF content in the cerebral cortex throughout the ages investigated. 
      Immunohistochemistry of hippocampal formation in 3-month-old mice revealed that 
      intensities of BDNF immunoreactivity in the dentate gyrus granule cell layer and 
      CA1 and CA3 pyramidal cell layers appeared diminished in mice fed the soft diet 
      compared with mice fed the hard diet. These results indicate that insufficient 
      mastication activity during development reduces BDNF protein levels in the 
      hippocampus and influences synaptic plasticity in this region.
FAU - Yamamoto, Tetsu
AU  - Yamamoto T
AD  - Department of Physiology, Tokyo Dental College, 1-2-2 Masago, Mihama-ku, Chiba, 
      Japan. ttyamamo@tdc.ac.jp
FAU - Hirayama, Akihiko
AU  - Hirayama A
FAU - Hosoe, Nobuo
AU  - Hosoe N
FAU - Furube, Masaru
AU  - Furube M
FAU - Hirano, Shusuke
AU  - Hirano S
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Bull Tokyo Dent Coll
JT  - The Bulletin of Tokyo Dental College
JID - 7505414
RN  - 0 (Brain-Derived Neurotrophic Factor)
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/analysis/*biosynthesis
MH  - *Diet
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression Regulation, Developmental
MH  - Hippocampus/*metabolism
MH  - Male
MH  - Mastication/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neuronal Plasticity/physiology
MH  - Synapses/physiology
EDAT- 2009/05/08 09:00
MHDA- 2009/05/19 09:00
CRDT- 2009/05/08 09:00
PHST- 2009/05/08 09:00 [entrez]
PHST- 2009/05/08 09:00 [pubmed]
PHST- 2009/05/19 09:00 [medline]
AID - JST.JSTAGE/tdcpublication/49.185 [pii]
AID - 10.2209/tdcpublication.49.185 [doi]
PST - ppublish
SO  - Bull Tokyo Dent Coll. 2008 Nov;49(4):185-90. doi: 10.2209/tdcpublication.49.185.