PMID- 19420928
OWN - NLM
STAT- MEDLINE
DCOM- 20090519
LR  - 20090507
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 124
IP  - 2
DP  - 2009
TI  - Repetitive DNA probe linked to sex chromosomes in hybrids between Neotropical 
      fish Leporinus macrocephalus and Leporinus elongatus (Characiformes, 
      Anostomidae).
PG  - 151-7
LID - 10.1159/000207523 [doi]
AB  - Interspecific hybrids of fish can offer important advances in the zootechnical 
      exploration of this animal group. However, hybrids can pose serious biological 
      risks to the environment and natural populations, making genetic characterization 
      and monitoring of hybrids produced in fish culture important concerns. In the 
      present study, individuals of the hybrid 'Piaupara' were characterized by 
      fluorescence in situ hybridization (FISH) using sex-specific probes to 
      differentiate the respective parental contributions from female Leporinus 
      macrocephalus (Piaucu) and male Leporinus elongatus (Piapara). The application of 
      this technique demonstrated a difference in hybridization patterns between males 
      and females of L. elongatus and L. macrocephalus, mainly due to the fact that the 
      NOR-bearing chromosomes of L. elongatus contain some homology with the probe. 
      This allowed us to detect a clear sex-specific distinction of the hybrid 
      'Piaupara', demonstrating that this technique is well-suited for the 
      characterization of these hybrids. Moreover, this technique offers an important 
      methodological advance for the study of diverse sex chromosome systems of 
      Leporinus, supplying valuable information about the origin and establishment of a 
      recently described sexual system in this genus.
CI  - Copyright 2009 S. Karger AG, Basel.
FAU - Hashimoto, D T
AU  - Hashimoto DT
AD  - Departamento de Ciencias Biologicas, Faculdade de Ciencias, Universidade Estadual 
      Paulista (UNESP), Campus de Bauru, Bauru, SP, Brazil.
FAU - Parise-Maltempi, P P
AU  - Parise-Maltempi PP
FAU - Laudicina, A
AU  - Laudicina A
FAU - Bortolozzi, J
AU  - Bortolozzi J
FAU - Senhorini, J A
AU  - Senhorini JA
FAU - Foresti, F
AU  - Foresti F
FAU - Porto-Foresti, F
AU  - Porto-Foresti F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090505
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - DNA Probes/*metabolism
MH  - Female
MH  - Fishes/*genetics
MH  - *Hybridization, Genetic
MH  - Male
MH  - Metaphase
MH  - Nucleolus Organizer Region
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Sex Chromosomes/*genetics
MH  - *Tropical Climate
EDAT- 2009/05/08 09:00
MHDA- 2009/05/20 09:00
CRDT- 2009/05/08 09:00
PHST- 2008/11/20 00:00 [received]
PHST- 2009/05/08 09:00 [entrez]
PHST- 2009/05/08 09:00 [pubmed]
PHST- 2009/05/20 09:00 [medline]
AID - 000207523 [pii]
AID - 10.1159/000207523 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2009;124(2):151-7. doi: 10.1159/000207523. Epub 2009 May 5.