PMID- 19420929
OWN - NLM
STAT- MEDLINE
DCOM- 20090519
LR  - 20211020
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Print)
IS  - 1424-8581 (Linking)
VI  - 124
IP  - 2
DP  - 2009
TI  - Studies on the short range spreading of the male specific lethal (MSL) complex on 
      the X chromosome in Drosophila.
PG  - 158-69
LID - 10.1159/000207524 [doi]
AB  - A series of autosomal insertions of chromosomal fragments derived from around the 
      X linked white eye color locus have been examined for male specific lethal (MSL) 
      complex binding using both immunostaining and fluorescence in situ hybridization 
      (FISH) techniques. The results show that the transposing elements (TEs) composed 
      of several genes in the white region (3C2-3C5) do not recruit the MSL complex 
      when inserted into an autosome. The same result is found for the Tp(1:3)wzh 
      insertion, a fragment of the X chromosome inserted into the third chromosome. Two 
      other insertions, Dp(1:2)w70h (3A7-3C2-3) and Dp(1:2)51b (3C2-3D6), which extend 
      more distally or proximally beyond the TE insertion, respectively, display a 
      binding pattern of the MSL complex at the autosomal location. These insertions 
      were also examined in females ectopically expressing MSL-2 and show similar 
      binding activity. In addition, the Tp(3:1)O5 transposition strain containing an 
      autosomal segment in the X chromosome was examined for spreading of the MSL 
      complex. Limited spreading of the MSL complex into autosomal regions was 
      indicated by immunostaining and FISH. This spreading was further confirmed by 
      chromatin immunoprecipitation of the MSL complex covering the autosomal 
      sequences.
CI  - Copyright 2009 S. Karger AG, Basel.
FAU - Sun, X
AU  - Sun X
AD  - Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.
FAU - Birchler, J A
AU  - Birchler JA
LA  - eng
GR  - R01 GM068042/GM/NIGMS NIH HHS/United States
GR  - R01 JM068042/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090505
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 0 (DNA Transposable Elements)
RN  - 0 (Drosophila Proteins)
RN  - 0 (ISWI protein)
RN  - 0 (Transcription Factors)
RN  - EC 3.6.1.- (Adenosine Triphosphatases)
SB  - IM
MH  - Adenosine Triphosphatases/genetics
MH  - Animals
MH  - Chromosome Breakage
MH  - DNA Transposable Elements/genetics
MH  - Drosophila/*genetics
MH  - Drosophila Proteins/*metabolism
MH  - Female
MH  - Genes, Insect
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mutation/genetics
MH  - Protein Binding
MH  - Protein Transport
MH  - *Sex Characteristics
MH  - Transcription Factors/genetics
MH  - X Chromosome/*genetics
PMC - PMC2919458
EDAT- 2009/05/08 09:00
MHDA- 2009/05/20 09:00
PMCR- 2010/05/01
CRDT- 2009/05/08 09:00
PHST- 2008/12/08 00:00 [accepted]
PHST- 2009/05/08 09:00 [entrez]
PHST- 2009/05/08 09:00 [pubmed]
PHST- 2009/05/20 09:00 [medline]
PHST- 2010/05/01 00:00 [pmc-release]
AID - 000207524 [pii]
AID - cgr0124-0158 [pii]
AID - 10.1159/000207524 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2009;124(2):158-69. doi: 10.1159/000207524. Epub 2009 May 
      5.