PMID- 19422882 OWN - NLM STAT- MEDLINE DCOM- 20090914 LR - 20090601 IS - 1872-7972 (Electronic) IS - 0304-3940 (Linking) VI - 459 IP - 2 DP - 2009 Aug 7 TI - Striatum brain-derived neurotrophic factor levels are decreased in dystrophin-deficient mice. PG - 66-8 LID - 10.1016/j.neulet.2009.04.065 [doi] AB - Brain dystrophin is enriched in the postsynaptic densities of pyramidal neurons specialized regions of the subsynaptic cytoskeletal network, which are critical for synaptic transmission and plasticity. Lack of dystrophin in brain structures have been involved with impaired cognitive functions. The brain-derived neurotrophic factor (BDNF) is a regulator of neuronal survival, fast synaptic transmission, and activity-dependent synaptic plasticity. The present study investigated BDNF protein levels by Elisa analysis in prefrontal cortex, cerebellum, hippocampus, striatum and cortex tissues from male dystrophic mdx (n=5) and normal C57BL10 mouse (n=5). We observed that the mdx mouse display diminution in BDNF levels in striatum (t=6.073; df=6; p=0.001), while a tendency of decrease in BDNF levels was observed in the prefrontal cortex region (t=1.962; df=6; p=0.096). The cerebellum (t=1.258; df=7; p=0.249), hippocampus (t=0.631; df=7; p=0.548) and cortex (t=0.572; df=7; p=0.586) showed no significant alterations as compared to wt mouse. In conclusion, we demonstrate that only striatum decreased BDNF levels compared with wild-type (wt) mouse, differently to the other areas of the brain. This dystrophin deficiency may be affecting BDNF levels in striatum and contributing, in part, in memory storage and restoring. FAU - Comim, Clarissa M AU - Comim CM AD - Laboratorio de Neurociencias and Instituto Nacional de Ciencia e Tecnologia Translacional em Medicina, Programa de Pos-Graduacao em Ciencias da Saude, Unidade Academica de Ciencias da Saude, Universidade do Extremo Sul Catarinense, SC, Brazil. FAU - Tuon, Lisiane AU - Tuon L FAU - Stertz, Laura AU - Stertz L FAU - Vainzof, Mariz AU - Vainzof M FAU - Kapczinski, Flavio AU - Kapczinski F FAU - Quevedo, Joao AU - Quevedo J LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090505 PL - Ireland TA - Neurosci Lett JT - Neuroscience letters JID - 7600130 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Dystrophin) SB - IM MH - Animals MH - Brain/*metabolism MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Cerebellum/metabolism MH - Cerebral Cortex/metabolism MH - Corpus Striatum/metabolism MH - Dystrophin/*deficiency MH - Enzyme-Linked Immunosorbent Assay MH - Hippocampus/metabolism MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Mice, Inbred mdx MH - Prefrontal Cortex/metabolism EDAT- 2009/05/09 09:00 MHDA- 2009/09/15 06:00 CRDT- 2009/05/09 09:00 PHST- 2009/03/03 00:00 [received] PHST- 2009/04/27 00:00 [revised] PHST- 2009/04/30 00:00 [accepted] PHST- 2009/05/09 09:00 [entrez] PHST- 2009/05/09 09:00 [pubmed] PHST- 2009/09/15 06:00 [medline] AID - S0304-3940(09)00594-1 [pii] AID - 10.1016/j.neulet.2009.04.065 [doi] PST - ppublish SO - Neurosci Lett. 2009 Aug 7;459(2):66-8. doi: 10.1016/j.neulet.2009.04.065. Epub 2009 May 5.