PMID- 19423639
OWN - NLM
STAT- MEDLINE
DCOM- 20091023
LR  - 20221207
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 23
IP  - 9
DP  - 2009 Sep
TI  - LPS induces KH-type splicing regulatory protein-dependent processing of 
      microRNA-155 precursors in macrophages.
PG  - 2898-908
LID - 10.1096/fj.09-131342 [doi]
AB  - The importance of post-transcriptional mechanisms for the regulation of the 
      homoeostasis of the immune system and the response to challenge by microorganisms 
      is becoming increasingly appreciated. We investigated the contribution of 
      microRNAs (miRNAs) to macrophage activation induced by lipopolysaccharide (LPS). 
      We first observed that Dicer knockout in bone marrow-derived macrophages (BMDMs) 
      increases the LPS-induced expression of some inflammation mediators. miRNA 
      microarray analysis in BMDMs revealed that LPS significantly induces the 
      expression of a single miRNA, miR-155, and this induction depends on enhanced 
      miR-155 maturation from its precursors. The single-strand RNA-binding protein 
      KH-type splicing regulatory protein (KSRP) binds to the terminal loop of miR-155 
      precursors and promotes their maturation. Both inhibition of miR-155 and KSRP 
      knockdown enhance the LPS-induced expression of select inflammation mediators, 
      and the effect of KSRP knockdown is reverted by mature miR-155. Our studies 
      unveil the existence of an LPS-dependent post-transcriptional regulation of 
      miR-155 biogenesis. Once induced, miR-155 finely tunes the expression of select 
      inflammation mediators in response to LPS.
FAU - Ruggiero, Tina
AU  - Ruggiero T
AD  - RNA Technology Laboratory, Centro Biotecnologie Avanzate, Genoa, Italy.
FAU - Trabucchi, Michele
AU  - Trabucchi M
FAU - De Santa, Francesca
AU  - De Santa F
FAU - Zupo, Simona
AU  - Zupo S
FAU - Harfe, Brian D
AU  - Harfe BD
FAU - McManus, Michael T
AU  - McManus MT
FAU - Rosenfeld, M Geoff
AU  - Rosenfeld MG
FAU - Briata, Paola
AU  - Briata P
FAU - Gherzi, Roberto
AU  - Gherzi R
LA  - eng
GR  - GFP04003/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090507
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Inflammation Mediators)
RN  - 0 (Khsrp protein, mouse)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn155 microRNA, mouse)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Trans-Activators)
RN  - EC 3.1.- (Endoribonucleases)
RN  - EC 3.1.26.3 (Dicer1 protein, mouse)
RN  - EC 3.1.26.3 (Ribonuclease III)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
SB  - IM
MH  - Animals
MH  - DEAD-box RNA Helicases/deficiency
MH  - Endoribonucleases/deficiency
MH  - Inflammation Mediators/metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages/*drug effects/metabolism
MH  - Mice
MH  - MicroRNAs/*metabolism
MH  - RNA Processing, Post-Transcriptional
MH  - RNA-Binding Proteins/*metabolism
MH  - Ribonuclease III
MH  - Trans-Activators/*metabolism
MH  - Transcriptional Activation
EDAT- 2009/05/09 09:00
MHDA- 2009/10/24 06:00
CRDT- 2009/05/09 09:00
PHST- 2009/05/09 09:00 [entrez]
PHST- 2009/05/09 09:00 [pubmed]
PHST- 2009/10/24 06:00 [medline]
AID - fj.09-131342 [pii]
AID - 10.1096/fj.09-131342 [doi]
PST - ppublish
SO  - FASEB J. 2009 Sep;23(9):2898-908. doi: 10.1096/fj.09-131342. Epub 2009 May 7.