PMID- 19426150
OWN - NLM
STAT- MEDLINE
DCOM- 20111223
LR  - 20211020
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 13
IP  - 9B
DP  - 2009 Sep
TI  - VEGFR2+PDGFRbeta+ circulating precursor cells participate in capillary 
      restoration after hyperoxia acute lung injury (HALI).
PG  - 3720-9
LID - 10.1111/j.1582-4934.2009.00785.x [doi]
AB  - The in vivo morphology and phenotype of circulating cells that spontaneously 
      contribute to new vessel formation in adults remain unclear. Here, we use 
      high-resolution imaging and flow cytometry to characterize the morphology and 
      phenotype of a distinct population of circulating mononuclear cells contributing 
      to spontaneous new vessel formation after hyperoxia acute lung injury (HALI). We 
      identify a subpopulation of myeloid (CD11b/Mac1(+)) haematopoietic cells 
      co-expressing vascular endothelial growth factor receptor 2 (VEGFR2) and platelet 
      derived growth factor receptor beta (PDGFRbeta). Moreover, we show that these 
      CD11b(+)VEGFR2(+)PDGFRbeta(+) circulating precursor cells (CPCs) contribute 
      structurally to the luminal surface of capillaries re-forming 2 weeks post-HALI. 
      This indicates that these myeloid CPCs may function, at least transiently, as 
      putative vascular precursors, and has important implications for capillary growth 
      and repair in injury and in pathologies of the lung and other organs.
FAU - Jones, Rosemary
AU  - Jones R
AD  - Department of Anesthesia and Critical Care, Massachusetts General Hospital and 
      Harvard Medical School, Boston, MA, USA.
FAU - Capen, Diane E
AU  - Capen DE
FAU - Jacobson, Margaretha
AU  - Jacobson M
FAU - Cohen, Kenneth S
AU  - Cohen KS
FAU - Scadden, David T
AU  - Scadden DT
FAU - Duda, Dan G
AU  - Duda DG
LA  - eng
GR  - R01 CA126642/CA/NCI NIH HHS/United States
GR  - R01 CA085140/CA/NCI NIH HHS/United States
GR  - P01 CA080124/CA/NCI NIH HHS/United States
GR  - R01 CA126642-01A1/CA/NCI NIH HHS/United States
GR  - R01 HL089252/HL/NHLBI NIH HHS/United States
GR  - R01 CA115767/CA/NCI NIH HHS/United States
GR  - P01 CA080124-08/CA/NCI NIH HHS/United States
GR  - R01 HL070866-04/HL/NHLBI NIH HHS/United States
GR  - R01 CA115767-03/CA/NCI NIH HHS/United States
GR  - R01 HL089252-01A1/HL/NHLBI NIH HHS/United States
GR  - R01 CA085140-09/CA/NCI NIH HHS/United States
GR  - R01 HL070866/HL/NHLBI NIH HHS/United States
GR  - K08 HL071938/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20090506
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (CD11b Antigen)
RN  - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor beta)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
SB  - IM
MH  - Acute Lung Injury/*pathology
MH  - Animals
MH  - CD11b Antigen/biosynthesis
MH  - Capillaries/*pathology
MH  - Flow Cytometry/methods
MH  - Hyperoxia/*pathology
MH  - Immunohistochemistry/methods
MH  - Kinetics
MH  - Lung/pathology
MH  - Male
MH  - Phenotype
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, Platelet-Derived Growth Factor beta/*biosynthesis
MH  - Vascular Endothelial Growth Factor Receptor-2/*biosynthesis
PMC - PMC2832073
MID - NIHMS121588
EDAT- 2009/05/12 09:00
MHDA- 2011/12/24 06:00
PMCR- 2009/09/01
CRDT- 2009/05/12 09:00
PHST- 2009/05/12 09:00 [entrez]
PHST- 2009/05/12 09:00 [pubmed]
PHST- 2011/12/24 06:00 [medline]
PHST- 2009/09/01 00:00 [pmc-release]
AID - JCMM785 [pii]
AID - 10.1111/j.1582-4934.2009.00785.x [doi]
PST - ppublish
SO  - J Cell Mol Med. 2009 Sep;13(9B):3720-9. doi: 10.1111/j.1582-4934.2009.00785.x. 
      Epub 2009 May 6.