PMID- 19427332
OWN - NLM
STAT- MEDLINE
DCOM- 20090811
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 160
IP  - 1-2
DP  - 2009 Sep
TI  - Development and validation of an immunogold chromatographic test for on-farm 
      detection of PRRSV.
PG  - 178-84
LID - 10.1016/j.jviromet.2009.04.034 [doi]
AB  - An immunochromatographic test strip was developed to detect porcine reproductive 
      and respiratory syndrome virus (PRRSV). The test uses two gold-labeled monoclonal 
      antibodies: D5 against recombinant nucleocapsid protein (rN) and E9 against 
      recombinant M protein (rM). In the test, PRRSV binds to a mixture of D5 and E9 
      labeled with colloidal gold; the complexes move through a membrane and are 
      captured by rabbit anti-rM and anti-rN antibodies at a test line, producing a 
      reddish-purple band because of the increased concentration of gold. Unbound 
      monoclonal antibodies move past the test line to be captured by goat anti-mouse 
      antibodies, producing a band at a control line. In samples without PRRSV or with 
      low virus concentration, a band appears only at the control line. A 
      crossover-trial demonstrated that the test strip was highly specific for PRRSV. 
      The test strip detection limit was between 7.8x10(3) and 1.6x10(4) TCID(50)/ml. 
      Analysis of 100 clinical samples indicated that the sensitivity, specificity, and 
      accuracy of the immunochromatographic test strip relative to reverse 
      transcription polymerase chain reaction (RT-PCR) were 97.0, 93.9, and 96.0%, 
      respectively. Because the test is simple and rapid, it can be used by an 
      unskilled person to detect PRRSV in the field.
FAU - Zhou, Sheng-hua
AU  - Zhou SH
AD  - Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 
      Harbin, China.
FAU - Cui, Shang-jin
AU  - Cui SJ
FAU - Chen, Chang-mu
AU  - Chen CM
FAU - Zhang, Fan-chao
AU  - Zhang FC
FAU - Li, Jun
AU  - Li J
FAU - Zhou, Shun
AU  - Zhou S
FAU - Oh, Jin-Sik
AU  - Oh JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20090507
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Monoclonal)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Chromatography/*methods
MH  - Immunohistochemistry/methods
MH  - Mice
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/*isolation & purification
MH  - Rabbits
MH  - Sensitivity and Specificity
MH  - Swine
EDAT- 2009/05/12 09:00
MHDA- 2009/08/12 09:00
CRDT- 2009/05/12 09:00
PHST- 2009/01/02 00:00 [received]
PHST- 2009/04/21 00:00 [revised]
PHST- 2009/04/23 00:00 [accepted]
PHST- 2009/05/12 09:00 [entrez]
PHST- 2009/05/12 09:00 [pubmed]
PHST- 2009/08/12 09:00 [medline]
AID - S0166-0934(09)00208-0 [pii]
AID - 10.1016/j.jviromet.2009.04.034 [doi]
PST - ppublish
SO  - J Virol Methods. 2009 Sep;160(1-2):178-84. doi: 10.1016/j.jviromet.2009.04.034. 
      Epub 2009 May 7.