PMID- 19427913 OWN - NLM STAT- MEDLINE DCOM- 20090928 LR - 20220317 IS - 1095-8355 (Electronic) IS - 1065-6995 (Linking) VI - 33 IP - 7 DP - 2009 Jul TI - Mesenchymal stem cells showed the highest potential for the regeneration of injured liver tissue compared with other subpopulations of the bone marrow. PG - 772-7 LID - 10.1016/j.cellbi.2009.04.023 [doi] AB - We have previously reported that bone marrow cells (BMCs) participate in the regeneration after liver injury. However, it is not established that this is the result of differentiation of hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) or the combination of both. We investigated the contribution of each cell fraction to the regenerative process. First, we confirmed that transplanted stem cells migrate directly to injured liver tissue without dispersing to other organs. Next, we divided green fluorescent protein (GFP)-expressing BMCs into three populations as mononuclear cells, MSCs and HSCs. We then compared the engraftment capacity after transplantation of each fraction of cells into liver-injured mice. Of these, the MSCs transplanted group showed the highest GFP fluorescence intensities in liver tissue by flow cytometry analysis and confocal microscopic observation. Furthermore, MSCs showed differentiation potential into hepatocytes when co-cultured with injured liver cells, which suggests that MSCs showed highest potential for the regeneration of injured liver tissue compared with those of the other two cell refractions. FAU - Cho, Kyung-Ah AU - Cho KA AD - Department of Microbiology, Ewha Womans University College of Medicine, Ewha Medical Research Center, Seoul, Republic of Korea. FAU - Ju, Sun-Young AU - Ju SY FAU - Cho, Su Jin AU - Cho SJ FAU - Jung, Yun-Jae AU - Jung YJ FAU - Woo, So-Youn AU - Woo SY FAU - Seoh, Ju-Young AU - Seoh JY FAU - Han, Ho-Seong AU - Han HS FAU - Ryu, Kyung-Ha AU - Ryu KH LA - eng GR - P30 DK067629/DK/NIDDK NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20090508 PL - England TA - Cell Biol Int JT - Cell biology international JID - 9307129 RN - 147336-22-9 (Green Fluorescent Proteins) RN - CL2T97X0V0 (Carbon Tetrachloride) SB - IM MH - Animals MH - Bone Marrow Cells/*cytology MH - Carbon Tetrachloride/toxicity MH - Chemical and Drug Induced Liver Injury MH - Coculture Techniques MH - Female MH - Green Fluorescent Proteins/genetics/metabolism MH - Hepatocytes/cytology MH - Liver Diseases/*therapy MH - Liver Regeneration/*physiology MH - Mesenchymal Stem Cells/*cytology MH - Mice MH - Mice, Inbred C57BL MH - Mice, Transgenic EDAT- 2009/05/12 09:00 MHDA- 2009/09/29 06:00 CRDT- 2009/05/12 09:00 PHST- 2008/12/14 00:00 [received] PHST- 2009/04/02 00:00 [revised] PHST- 2009/04/23 00:00 [accepted] PHST- 2009/05/12 09:00 [entrez] PHST- 2009/05/12 09:00 [pubmed] PHST- 2009/09/29 06:00 [medline] AID - S1065-6995(09)00105-X [pii] AID - 10.1016/j.cellbi.2009.04.023 [doi] PST - ppublish SO - Cell Biol Int. 2009 Jul;33(7):772-7. doi: 10.1016/j.cellbi.2009.04.023. Epub 2009 May 8.