PMID- 19427914
OWN - NLM
STAT- MEDLINE
DCOM- 20090928
LR  - 20171116
IS  - 1095-8355 (Electronic)
IS  - 1065-6995 (Linking)
VI  - 33
IP  - 7
DP  - 2009 Jul
TI  - In vitro generation of murine myeloid dendritic cells from CD34-positive 
      precursors.
PG  - 778-84
LID - 10.1016/j.cellbi.2009.04.022 [doi]
AB  - Dendritic cells (DCs) link the innate and adaptive immune system. Currently, 
      murine DCs for cell biology investigations are developed from MHC class 
      II-negative bone marrow (BM) precursor cells, non-depleted BM cells or BM 
      monocytes in the presence of granulocyte-macrophage colony-stimulating factor 
      (GM-CSF). Here we demonstrate an isolation procedure of functionally intact 
      myeloid CD11c(+) CD11b(+) DCs derived from murine CD34-positive precursors. DCs 
      derived from CD34(+) cells show functional internalization, maturation, cytokine 
      secretion, MHC-restricted antigen presentation, and MHCII retrograde transport of 
      antigens from the lysosomes to the cell surface. In comparison to the established 
      method, the advantages of this isolation procedure are a shorter cultivation 
      period, a superior transfection efficiency, the yield of a purer and more 
      homogeneous population of immature DCs, and less consumption of cell culture 
      medium and GM-CSF. The new isolation procedure and the functional quality of 
      CD34(+) cell-derived murine myeloid DCs make them ideally suited for immunology 
      and cell biology studies.
FAU - Stephen, Tom-Li
AU  - Stephen TL
AD  - Institute for Medical Microbiology, Immunology and Hygiene, Medical Center, 
      University of Cologne, Cologne, Germany.
FAU - Harms, Fabian
AU  - Harms F
FAU - Fabri, Mario
AU  - Fabri M
FAU - Flenner, Eva
AU  - Flenner E
FAU - Bessler, Martina
AU  - Bessler M
FAU - Hafke, Helena
AU  - Hafke H
FAU - Meemboor, Sonja
AU  - Meemboor S
FAU - Kalka, Christoph
AU  - Kalka C
FAU - Kalka-Moll, Wiltrud
AU  - Kalka-Moll W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090508
PL  - England
TA  - Cell Biol Int
JT  - Cell biology international
JID - 9307129
RN  - 0 (Antigens, CD34)
RN  - 0 (CD11b Antigen)
RN  - 0 (CD11c Antigen)
RN  - 0 (Recombinant Proteins)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigens, CD34/*metabolism
MH  - Bone Marrow Cells/cytology/drug effects
MH  - CD11b Antigen/metabolism
MH  - CD11c Antigen/metabolism
MH  - Cell Culture Techniques
MH  - Dendritic Cells/*cytology/drug effects/immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phenotype
MH  - Recombinant Proteins
MH  - Transfection
EDAT- 2009/05/12 09:00
MHDA- 2009/09/29 06:00
CRDT- 2009/05/12 09:00
PHST- 2009/02/02 00:00 [received]
PHST- 2009/03/19 00:00 [revised]
PHST- 2009/04/23 00:00 [accepted]
PHST- 2009/05/12 09:00 [entrez]
PHST- 2009/05/12 09:00 [pubmed]
PHST- 2009/09/29 06:00 [medline]
AID - S1065-6995(09)00106-1 [pii]
AID - 10.1016/j.cellbi.2009.04.022 [doi]
PST - ppublish
SO  - Cell Biol Int. 2009 Jul;33(7):778-84. doi: 10.1016/j.cellbi.2009.04.022. Epub 
      2009 May 8.