PMID- 19428103
OWN - NLM
STAT- MEDLINE
DCOM- 20091123
LR  - 20090923
IS  - 1873-5835 (Electronic)
IS  - 0145-2126 (Linking)
VI  - 33
IP  - 12
DP  - 2009 Dec
TI  - Characterization of chronic lymphocytic leukemia/small lymphocytic lymphoma 
      (CLL/SLL) in Shanghai, China: molecular and cytogenetic characteristics, IgV gene 
      restriction and hypermutation patterns.
PG  - 1599-603
LID - 10.1016/j.leukres.2008.12.021 [doi]
AB  - The clinical, cytogenetic and molecular features of chronic lymphocytic 
      leukemia/small lymphocytic lymphoma (CLL/SLL), a disease previously considered to 
      be rare in Asia, were examined in consecutive series of 70 cases diagnosed by our 
      laboratory over a 30-month period. Clonal abnormalities were observed in 80% of 
      CLL/SLL cases using a combination of conventional cytogenetic and fluorescence in 
      situ hybridization (FISH) analysis. Those involving 14q32/IGH were the most 
      frequent (24 cases), followed by trisomy 12 and 11q abnormalities. IgV(H) gene 
      usage was non-random with over-representation of V(H)4-34, V(H)3-23 and a 
      previously unreported increase in V(H)3-48 gene use. Somatic hypermutation (SHM) 
      of IgV(H) germline sequences was observed in 56.5% of cases with stereotyped 
      patterns of SHM observed in V(H)4-34 heavy chain complimentary-determining 
      (HCDR1) and framework region CFR2 sequences. These findings in a Chinese 
      population suggest subtle geographical differences in IgV(H) gene usage while the 
      remarkably specific pattern of SHM suggest that a relatively limited set of 
      antigens may be involved in the development of this disease worldwide. IgV(H) 
      gene mutation status was a significant predictor of initial survival in CLL/SLL. 
      However, an influence of karyotype on prognosis was not observed.
FAU - Irons, Richard D
AU  - Irons RD
AD  - Fudan-Cinpathogen Clinical and Molecular Research Center, Institutes of 
      Biomedical Sciences, Fudan University, Shanghai, China. 
      Richard.irons@ucdenver.edu
FAU - Le, Anh
AU  - Le A
FAU - Bao, Liming
AU  - Bao L
FAU - Zhu, Xiongzeng
AU  - Zhu X
FAU - Ryder, John
AU  - Ryder J
FAU - Wang, Xiao Qin
AU  - Wang XQ
FAU - Ji, Meirong
AU  - Ji M
FAU - Chen, Yan
AU  - Chen Y
FAU - Wu, Xichun
AU  - Wu X
FAU - Lin, Guowei
AU  - Lin G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090509
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (DNA Primers)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - China
MH  - DNA Primers
MH  - Female
MH  - *Genes, Immunoglobulin
MH  - Humans
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Mutation
EDAT- 2009/05/12 09:00
MHDA- 2009/12/16 06:00
CRDT- 2009/05/12 09:00
PHST- 2008/12/22 00:00 [received]
PHST- 2008/12/22 00:00 [revised]
PHST- 2008/12/29 00:00 [accepted]
PHST- 2009/05/12 09:00 [entrez]
PHST- 2009/05/12 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
AID - S0145-2126(09)00005-8 [pii]
AID - 10.1016/j.leukres.2008.12.021 [doi]
PST - ppublish
SO  - Leuk Res. 2009 Dec;33(12):1599-603. doi: 10.1016/j.leukres.2008.12.021. Epub 2009 
      May 9.