PMID- 19428745
OWN - NLM
STAT- MEDLINE
DCOM- 20090701
LR  - 20090511
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 142
IP  - 1-2
DP  - 2009 Jun
TI  - Characterization of the Chaetoceros salsugineum nuclear inclusion virus coat 
      protein gene.
PG  - 127-33
LID - 10.1016/j.virusres.2009.01.021 [doi]
AB  - Analysis of the genome of Chaetoceros salsugineum nuclear inclusion virus (CsNIV) 
      revealed the presence of six putative open reading frames (ORFs) in the genome. 
      We further characterized ORF3, which encodes a putative coat protein. Polymerase 
      chain reaction (PCR) using ORF3 gene-specific primers amplified a single DNA band 
      nearly 1.2kb. This amplified product was gel-purified, cloned, sequenced, and 
      expressed in Escherichia coli. Specific antiserum was raised against the 
      recombinant protein and used for Western blotting to test whether the ORF3 
      protein is the CsNIV coat protein. One major CsNIV protein of approximately 46kDa 
      reacted positively with the antiserum, suggesting that this antiserum is specific 
      for the CsNIV coat protein. Matrix-assisted laser desorption/ionization 
      time-of-flight (MALDI-TOF) analysis of the 46kDa structural band revealed 14 
      peptide sequences that matched the ORF3 regions of CsNIV. The expression of ORF3 
      in host cells was examined by constructing a cDNA library of CsNIV-infected 
      cells. Nucleotide sequences of the cDNA clones were complementary to various 
      regions of both CsNIV ORF3 and ORF4; however, no clones containing only the ORF3 
      region were identified. Also, Northern blotting revealed a single 2.5-kb 
      transcript, indicating that ORF3 could be transcribed together with ORF4.
FAU - Park, Yunjung
AU  - Park Y
AD  - Department of Microbiology, Pukyong National University, 599-1, Daeyeon 3-Dong, 
      Nam-Gu, Busan 608-737, South Korea.
FAU - Jung, Sang-Eun
AU  - Jung SE
FAU - Tomaru, Yuji
AU  - Tomaru Y
FAU - Choi, Woobong
AU  - Choi W
FAU - Kim, Yoonhee
AU  - Kim Y
FAU - Mizumoto, Hiroyuki
AU  - Mizumoto H
FAU - Nagasaki, Keizo
AU  - Nagasaki K
FAU - Choi, Tae-Jin
AU  - Choi TJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090212
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Capsid Proteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Capsid Proteins/chemistry/*genetics
MH  - Diatoms/*virology
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Viral Proteins/chemistry/genetics
MH  - Viruses/chemistry/*genetics/*isolation & purification
EDAT- 2009/05/12 09:00
MHDA- 2009/07/02 09:00
CRDT- 2009/05/12 09:00
PHST- 2008/08/31 00:00 [received]
PHST- 2008/12/16 00:00 [revised]
PHST- 2009/01/31 00:00 [accepted]
PHST- 2009/05/12 09:00 [entrez]
PHST- 2009/05/12 09:00 [pubmed]
PHST- 2009/07/02 09:00 [medline]
AID - S0168-1702(09)00045-8 [pii]
AID - 10.1016/j.virusres.2009.01.021 [doi]
PST - ppublish
SO  - Virus Res. 2009 Jun;142(1-2):127-33. doi: 10.1016/j.virusres.2009.01.021. Epub 
      2009 Feb 12.