PMID- 19428752
OWN - NLM
STAT- MEDLINE
DCOM- 20090701
LR  - 20191210
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 142
IP  - 1-2
DP  - 2009 Jun
TI  - Ostreid herpes virus 1 infection in families of the Pacific oyster, Crassostrea 
      gigas, during a summer mortality outbreak: differences in viral DNA detection and 
      quantification using real-time PCR.
PG  - 181-7
LID - 10.1016/j.virusres.2009.02.013 [doi]
AB  - Ostreid herpes virus 1 (OsHV-1) infections, notably reported in Europe and the 
      USA, are closely associated with significant mortalities of the Pacific oyster, 
      Crassostrea gigas, especially during its early stages of life. In summer 2006, we 
      monitored mortality by strict daily verification of three full-sib families of 
      oysters reared under common conditions. We quantified OsHV-1 using real-time PCR 
      in dead and living individuals during and after a mortality event. Mortality 
      events were severe and brief, but significantly different between tested families 
      (cumulative mortality ranging from 1.2 to 49%). Real-time PCR assays revealed 
      different viral DNA loads in dead individuals from different families (P<0.001). 
      Moreover, the mean level of infection among families was correlated with 
      mortality (P<0.05). Living oysters showed a significantly lower amount of viral 
      DNA compared with dead ones. This is the first experiment showing the daily 
      changes of individual OsHV-1 DNA load during a mortality outbreak. Our results 
      also support the previously reported high genetic basis underlying the variance 
      of resistance of Pacific oyster to summer mortality, suggesting that there might 
      be a possibility to improve resistance to OsHV-1 by selective breeding.
FAU - Sauvage, Christopher
AU  - Sauvage C
AD  - Ifremer, Laboratoire de Genetique et Pathologie, Avenue Mus de Loup, 17390 La 
      Tremblade, France.
FAU - Pepin, Jean Francois
AU  - Pepin JF
FAU - Lapegue, Sylvie
AU  - Lapegue S
FAU - Boudry, Pierre
AU  - Boudry P
FAU - Renault, Tristan
AU  - Renault T
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090306
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Crassostrea/*virology
MH  - DNA, Viral/*genetics
MH  - Herpesviridae/genetics/*isolation & purification
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/*methods
MH  - Seasons
MH  - Shellfish/*virology
EDAT- 2009/05/12 09:00
MHDA- 2009/07/02 09:00
CRDT- 2009/05/12 09:00
PHST- 2008/12/18 00:00 [received]
PHST- 2009/02/20 00:00 [revised]
PHST- 2009/02/20 00:00 [accepted]
PHST- 2009/05/12 09:00 [entrez]
PHST- 2009/05/12 09:00 [pubmed]
PHST- 2009/07/02 09:00 [medline]
AID - S0168-1702(09)00090-2 [pii]
AID - 10.1016/j.virusres.2009.02.013 [doi]
PST - ppublish
SO  - Virus Res. 2009 Jun;142(1-2):181-7. doi: 10.1016/j.virusres.2009.02.013. Epub 
      2009 Mar 6.