PMID- 19428786
OWN - NLM
STAT- MEDLINE
DCOM- 20090715
LR  - 20211020
IS  - 1872-9754 (Electronic)
IS  - 0197-0186 (Print)
IS  - 0197-0186 (Linking)
VI  - 54
IP  - 7
DP  - 2009 Jun
TI  - Activation of EP2 prostanoid receptors in human glial cell lines stimulates the 
      secretion of BDNF.
PG  - 439-46
LID - 10.1016/j.neuint.2009.01.018 [doi]
AB  - Prostaglandin E(2) (PGE(2)) is produced at high levels in the injured central 
      nervous system, where it is generally considered a cytotoxic mediator of 
      inflammation. The cellular actions of PGE(2) are mediated by G-protein signaling 
      activated by prostanoid receptors termed EP(1), EP(2), EP(3) and EP(4). Recent 
      studies have implicated the EP(2) prostanoid receptor to be in apparently 
      conflicting roles promoting neuronal death in some model systems and the survival 
      of neurons in others. Here we show that treatment of immortalized human microglia 
      and CCF-STTG1 astrocytes with either PGE(2) or the EP(2) selective agonist 
      butaprost stimulates the release of brain-derived neurotrophic factor (BDNF). 
      Both cell lines express mRNA for the EP(2) receptor, whereas transcripts for the 
      other subtypes are not detected. Pharmacological studies using PGE(2) and 
      modulators of cyclic AMP signaling implicate this pathway in PGE(2)-stimulated 
      BDNF release. These results indicate that EP(2) prostanoid receptor activation 
      induces BDNF secretion through stimulation of cyclic AMP dependent signaling. Our 
      findings provide a mechanism by which endogenous PGE(2) might contribute to 
      either neurotoxicity or neuroprotection in the injured brain via the induction of 
      BDNF release from microglial cells and astrocytes.
FAU - Hutchinson, Anthony J
AU  - Hutchinson AJ
AD  - Program in Neuroscience, The University of Arizona, College of Pharmacy, 
      Gould-Simpson 611, PO Box 210077, Tucson, AZ 85721, USA.
FAU - Chou, Chih-Ling
AU  - Chou CL
FAU - Israel, Davelene D
AU  - Israel DD
FAU - Xu, Wei
AU  - Xu W
FAU - Regan, John W
AU  - Regan JW
LA  - eng
GR  - R01 EY011291/EY/NEI NIH HHS/United States
GR  - R01 EY011291-10/EY/NEI NIH HHS/United States
GR  - EY11291/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090206
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Prostaglandin E)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
RN  - F5TD010360 (Alprostadil)
RN  - HP16WVP23Y (butaprost)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Adenylyl Cyclases/metabolism
MH  - Alprostadil/analogs & derivatives/pharmacology
MH  - Astrocytes/metabolism
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Line
MH  - Cyclic AMP/biosynthesis
MH  - Cyclic AMP Response Element-Binding Protein/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Dinoprostone/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Luciferases/genetics
MH  - Neuroglia/drug effects/*metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Receptors, Prostaglandin E/*agonists
MH  - Transfection
PMC - PMC2717006
MID - NIHMS111976
EDAT- 2009/05/12 09:00
MHDA- 2009/07/16 09:00
PMCR- 2010/06/01
CRDT- 2009/05/12 09:00
PHST- 2008/09/04 00:00 [received]
PHST- 2008/12/27 00:00 [revised]
PHST- 2009/01/22 00:00 [accepted]
PHST- 2009/05/12 09:00 [entrez]
PHST- 2009/05/12 09:00 [pubmed]
PHST- 2009/07/16 09:00 [medline]
PHST- 2010/06/01 00:00 [pmc-release]
AID - S0197-0186(09)00034-5 [pii]
AID - 10.1016/j.neuint.2009.01.018 [doi]
PST - ppublish
SO  - Neurochem Int. 2009 Jun;54(7):439-46. doi: 10.1016/j.neuint.2009.01.018. Epub 
      2009 Feb 6.