PMID- 19430298
OWN - NLM
STAT- MEDLINE
DCOM- 20090710
LR  - 20090525
IS  - 1533-4066 (Electronic)
IS  - 1052-9551 (Linking)
VI  - 18
IP  - 2
DP  - 2009 Jun
TI  - Aberrations of chromosome 13q in gastrointestinal stromal tumors: analysis of 91 
      cases by fluorescence in situ hybridization (FISH).
PG  - 72-80
LID - 10.1097/PDM.0b013e318181fa1f [doi]
AB  - The clinical behavior of gastrointestinal stromal tumors (GISTs) ranges from 
      benign to malignant. Recent studies suggest that loss of 13q could be correlated 
      with GIST progression. Our objectives were: (1) to detect chromosome 13q 
      aberrations and determine the corresponding gene status in GISTs; and (2) to 
      assess potential roles of 13q aberrations in GIST by correlating various 13q 
      aberrations with various histologic parameters and disease-free survival in a 
      group of GIST patients. Ninety-one cases of primary GISTs in Chinese patients 
      were studied by dual color fluorescence in situ hybridization (FISH), through use 
      of a panel of bacterial artificial chromosome clones RP11-685I15, RP11-352N7, and 
      RP11-505F3 covering the Rb, RFP2, KCNRG, and KLF5 genes, respectively. Loss of 
      RP11-685I15 was detected in 17/91 (18.7%) cases, loss of RP11-352N7 in 11/91 
      (12.1%) cases, and loss of RP11-505F3 in 5/91 (5.5%) cases. Chromosome 13 
      polysomy was observed in 22/91 (24.2%) cases. The frequency of RP11-685I15 
      deletion was positively correlated with tumor risk (P=0.0460). The frequency of 
      RP11-352N7 deletion, RP11-505F3 deletion, and chromosome 13 polysomy tended to be 
      higher in the high-risk GISTs. Shorter disease-free survival was significantly 
      associated with RP11-352N7 deletion (P=0.0361) and high-risk grade (P=0.0003). 
      Chromosome 13 instability of GISTs may play a role in tumor progression. Loss of 
      13q, especially loss of Rb, RFP2, KCNRG, and KLF5 genes are frequent events in 
      high-risk GISTs. Loss of 13q may be associated with tumor progression.
FAU - Zhou, Weixun
AU  - Zhou W
AD  - Department of Pathology, Peking Union Medical College Hospital, Chinese Academy 
      of Medical Sciences, Beijing, China.
FAU - Zeng, Xuan
AU  - Zeng X
FAU - Liu, Tonghua
AU  - Liu T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Diagn Mol Pathol
JT  - Diagnostic molecular pathology : the American journal of surgical pathology, part 
      B
JID - 9204924
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aneuploidy
MH  - China
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - *Chromosomes, Human, Pair 13
MH  - Female
MH  - Gastrointestinal Stromal Tumors/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Young Adult
EDAT- 2009/05/12 09:00
MHDA- 2009/07/11 09:00
CRDT- 2009/05/12 09:00
PHST- 2009/05/12 09:00 [entrez]
PHST- 2009/05/12 09:00 [pubmed]
PHST- 2009/07/11 09:00 [medline]
AID - 10.1097/PDM.0b013e318181fa1f [doi]
PST - ppublish
SO  - Diagn Mol Pathol. 2009 Jun;18(2):72-80. doi: 10.1097/PDM.0b013e318181fa1f.