PMID- 19430914
OWN - NLM
STAT- MEDLINE
DCOM- 20100106
LR  - 20220321
IS  - 1573-6857 (Electronic)
IS  - 0016-6707 (Linking)
VI  - 137
IP  - 3
DP  - 2009 Dec
TI  - Chromosomal localization of 18S and 5S rDNA using FISH in the genus Tor (Pisces, 
      Cyprinidae).
PG  - 245-52
LID - 10.1007/s10709-009-9367-x [doi]
AB  - Dual color fluorescence in situ hybridization (FISH) was performed to study the 
      simultaneous chromosomal localization of 18S and 5S ribosomal genes in the genus 
      Tor for the first time. The 18S and 5S rDNAs in four Tor species were amplified, 
      sequenced and mapped on the metaphase chromosomes. The number and distribution of 
      18S and 5S rDNA clusters were examined on metaphase chromosome spreads using 
      FISH. The specimens of T. chelynoides, T. putitora and T. progeneius showed six 
      bright fluorescent signals of 18S rDNA and T. tor exhibited ten such signals. The 
      5S rDNA signals were present only on one pair of chromosomes in all the four Tor 
      species. Ag-NORs were observed on two pairs of chromosomes in T. chelynoides, T. 
      putitora, T. progeneius and four pairs in T. tor. Comparison of the observed 18S 
      rDNA FISH signals and Ag-NORs strongly suggested a possible inactivation of NORs 
      localized at the telomeres of a subtelocentric and telocentric chromosome pairs 
      in all four species. The 5S rDNA contained an identical 120 bp long coding region 
      and 81 bp long highly divergent non-transcribed spacers in all species examined. 
      18S and 5S rDNA sequencing and chromosomal localization can be a useful genetic 
      marker in species identification as well as phylogenetic and evolutionary 
      studies.
FAU - Singh, Mamta
AU  - Singh M
AD  - National Bureau of Fish Genetic Resources, Canal Ring Road, P.O- Dilkusha, 
      Lucknow, 226 002, Uttar Pradesh, India.
FAU - Kumar, Ravindra
AU  - Kumar R
FAU - Nagpure, N S
AU  - Nagpure NS
FAU - Kushwaha, B
AU  - Kushwaha B
FAU - Gond, Indramani
AU  - Gond I
FAU - Lakra, W S
AU  - Lakra WS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20090509
PL  - Netherlands
TA  - Genetica
JT  - Genetica
JID - 0370740
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Chromosome Mapping/*methods
MH  - Cyprinidae/*genetics
MH  - DNA, Ribosomal/analysis/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Sequence Data
MH  - RNA, Ribosomal, 18S/*genetics
MH  - RNA, Ribosomal, 5S/*genetics
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
EDAT- 2009/05/12 09:00
MHDA- 2010/01/07 06:00
CRDT- 2009/05/12 09:00
PHST- 2008/08/30 00:00 [received]
PHST- 2009/04/24 00:00 [accepted]
PHST- 2009/05/12 09:00 [entrez]
PHST- 2009/05/12 09:00 [pubmed]
PHST- 2010/01/07 06:00 [medline]
AID - 10.1007/s10709-009-9367-x [doi]
PST - ppublish
SO  - Genetica. 2009 Dec;137(3):245-52. doi: 10.1007/s10709-009-9367-x. Epub 2009 May 
      9.