PMID- 19431195
OWN - NLM
STAT- MEDLINE
DCOM- 20090909
LR  - 20090616
IS  - 1098-2264 (Electronic)
IS  - 1045-2257 (Linking)
VI  - 48
IP  - 8
DP  - 2009 Aug
TI  - Identification of novel HMGA2 fusion sequences in lipoma: evidence that deletion 
      of let-7 miRNA consensus binding site 1 in the HMGA2 3' UTR is not critical for 
      HMGA2 transcriptional upregulation.
PG  - 673-8
LID - 10.1002/gcc.20674 [doi]
AB  - Lipoma is a benign tumor composed of mature adipocytes and one of the most common 
      mesenchymal tumors seen in adults. Rearrangement of HMGA2 in chromosome band 
      12q15 has been found in approximately 60-70% of ordinary lipomas with cytogenetic 
      abnormalities. Herein, we report two novel HMGA2 fusion sequences in lipomas with 
      chromosome 12 rearrangements. Cytogenetic studies showed 12q abnormalities in 
      both cases, and fluorescence in situ hybridization (FISH) confirmed the 
      involvement of HMGA2 in each instance. Rapid amplification of cDNA ends (RACE) 
      PCR experiments revealed that one lipoma contained a fusion of the HMGA2 3' 
      untranslated region (UTR) to a genomic area downstream of the DYRK2 locus on 
      12q15; the second lipoma showed a fusion of the HMGA2 3' UTR to a genomic 
      sequence upstream of the DCN locus on 12q21. In both instances the breakpoint on 
      HMGA2 occurred downstream to let-7 miRNA (microRNA) consensus binding site (CBS) 
      1. These two and several other previously reported tumors containing HMGA2 3' UTR 
      rearrangements show breakpoints after let-7 miRNA CBS 1, which suggests that the 
      elimination of this miRNA binding site is not critical for driving HMGA2 
      transcriptional upregulation.
FAU - Wang, Xiaoke
AU  - Wang X
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 
      55905, USA.
FAU - Hulshizer, Rachael L
AU  - Hulshizer RL
FAU - Erickson-Johnson, Michele R
AU  - Erickson-Johnson MR
FAU - Flynn, Heather C
AU  - Flynn HC
FAU - Jenkins, Robert B
AU  - Jenkins RB
FAU - Lloyd, Ricardo V
AU  - Lloyd RV
FAU - Oliveira, Andre M
AU  - Oliveira AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (HMGA2 Protein)
RN  - 0 (MicroRNAs)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (mirnlet7 microRNA, human)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Consensus Sequence/genetics
MH  - Female
MH  - Gene Deletion
MH  - HMGA2 Protein/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lipoma/*genetics
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Molecular Sequence Data
MH  - Oncogene Proteins, Fusion/*genetics
MH  - Polymerase Chain Reaction
MH  - Transcription, Genetic
MH  - *Up-Regulation
EDAT- 2009/05/12 09:00
MHDA- 2009/09/10 06:00
CRDT- 2009/05/12 09:00
PHST- 2009/05/12 09:00 [entrez]
PHST- 2009/05/12 09:00 [pubmed]
PHST- 2009/09/10 06:00 [medline]
AID - 10.1002/gcc.20674 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2009 Aug;48(8):673-8. doi: 10.1002/gcc.20674.