PMID- 19432006
OWN - NLM
STAT- MEDLINE
DCOM- 20090623
LR  - 20090512
IS  - 0005-2086 (Print)
IS  - 0005-2086 (Linking)
VI  - 53
IP  - 1
DP  - 2009 Mar
TI  - Development of a real-time polymerase chain reaction assay for the simultaneous 
      detection of Mycoplasma gallisepticum and Mycoplasma synoviae under industry 
      conditions.
PG  - 73-7
AB  - In this research we developed a real-time SYBR green assay to detect both 
      Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in a single reaction. 
      A total of 30,000 samples from broiler breeder flocks were screened using 
      traditional serology (plate agglutination, enzyme-linked immunosorbent assay, 
      hemagglutination inhibition) and polymerase chain reaction (PCR; traditional and 
      real-time). It was determined that the real-time SYBR green PCR assay developed 
      in this research was more rapid than all three methods tested and more sensitive 
      and specific than culturing or serology. The SYBR green assay was optimized and 
      could detect as few as 30 template copies of DNA per sample. In addition, the 
      SYBR green assay was less expensive than traditional culturing and serology. MG 
      and MS are infectious bacteria that can rapidly spread and infect commercial 
      chicken flocks. These diseases can cause a significant loss to the poultry 
      industry and especially to broiler breeders because infected flocks are destroyed 
      under the National Poultry Improvement Plan MG and MS clean programs. The 
      real-time SYBR green assay developed in this research has the potential to reduce 
      the time it takes to reach a correct diagnosis and to arrest outbreaks of MG and 
      MS.
FAU - Jarquin, Robin
AU  - Jarquin R
AD  - Research and Development, Cobb-Vantress Incorporated, P.O. Box 1030, Siloam 
      Springs, AR 72761, USA. robin.jarquin@cobb-vantress.com
FAU - Schultz, Joseph
AU  - Schultz J
FAU - Hanning, Irene
AU  - Hanning I
FAU - Ricke, Steven C
AU  - Ricke SC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Avian Dis
JT  - Avian diseases
JID - 0370617
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - Animals
MH  - *Chickens
MH  - DNA, Bacterial/classification/isolation & purification
MH  - Mycoplasma Infections/*veterinary
MH  - Mycoplasma gallisepticum/*isolation & purification
MH  - Mycoplasma synoviae/*isolation & purification
MH  - Nucleic Acid Denaturation
MH  - Polymerase Chain Reaction/methods/*veterinary
MH  - Poultry Diseases/*microbiology
EDAT- 2009/05/13 09:00
MHDA- 2009/06/24 09:00
CRDT- 2009/05/13 09:00
PHST- 2009/05/13 09:00 [entrez]
PHST- 2009/05/13 09:00 [pubmed]
PHST- 2009/06/24 09:00 [medline]
AID - 10.1637/8445-080808-Reg.1 [doi]
PST - ppublish
SO  - Avian Dis. 2009 Mar;53(1):73-7. doi: 10.1637/8445-080808-Reg.1.