PMID- 19432011
OWN - NLM
STAT- MEDLINE
DCOM- 20090623
LR  - 20220408
IS  - 0005-2086 (Print)
IS  - 0005-2086 (Linking)
VI  - 53
IP  - 1
DP  - 2009 Mar
TI  - The development of diagnostic real-time TaqMan PCRs for the four pathogenic avian 
      mycoplasmas.
PG  - 103-7
AB  - Four avian mycoplasmas are commonly recognized as poultry pathogens: Mycoplasma 
      gallisepticum (MG), Mycoplasma synoviae (MS), Mycoplasma meleagridis (MM), and 
      Mycoplasma iowae (MI). The avian mycoplasmas are associated with respiratory 
      disease, synovitis and arthritis, poor performance, skeletal deformities, and 
      embryo mortality. Three main approaches are used for the diagnosis of avian 
      mycoplasmosis: isolation and identification, detection of antibodies, and 
      molecular detection of the organism's nucleic acid by PCR. In recent years 
      real-time PCR technology has revolutionized the way clinical microbiology 
      laboratories diagnose infectious diseases, but so far only a limited number of 
      diagnostic real-time PCRs have been proposed for avian mycoplasma diagnostics. We 
      developed a complete set of reliable diagnostic real-time TaqMan PCR assays for 
      the four pathogenic avian mycoplasmas. The selected genomic targets of the 
      developed assays were species specific and intraspecifically conserved and 
      included the 16S-23S intergenic spacer region of MS and MM, the upstream region 
      to the 16S ribosomal DNA of MI, and highly conserved foci of the mgc2 gene of MG. 
      The four assays were demonstrated to be highly specific and sensitive to their 
      target avian mycoplasma, with detection limits of one copy per reaction mix for 
      the MG assay and 10 copies per reaction mix for the MS, MM, and MI assays. We 
      believe that the incorporation of the developed assays in avian mycoplasma 
      diagnostics will contribute to the accuracy, efficiency, and feasibility of 
      diagnosis of these pathogens.
FAU - Raviv, Ziv
AU  - Raviv Z
AD  - Department of Population Health, Poultry Diagnostic and Research Center, 
      University of Georgia, Athens, GA 30602-4875, USA. zraviv@uga.edu
FAU - Kleven, Stanley H
AU  - Kleven SH
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Avian Dis
JT  - Avian diseases
JID - 0370617
SB  - IM
MH  - Animals
MH  - Genome, Bacterial
MH  - Mycoplasma/*classification/*isolation & purification
MH  - Polymerase Chain Reaction/*methods
MH  - Poultry
MH  - Poultry Diseases/diagnosis/microbiology
MH  - Sensitivity and Specificity
EDAT- 2009/05/13 09:00
MHDA- 2009/06/24 09:00
CRDT- 2009/05/13 09:00
PHST- 2009/05/13 09:00 [entrez]
PHST- 2009/05/13 09:00 [pubmed]
PHST- 2009/06/24 09:00 [medline]
AID - 10.1637/8469-091508-Reg.1 [doi]
PST - ppublish
SO  - Avian Dis. 2009 Mar;53(1):103-7. doi: 10.1637/8469-091508-Reg.1.