PMID- 19434662
OWN - NLM
STAT- MEDLINE
DCOM- 20091116
LR  - 20181201
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 3
IP  - 5
DP  - 2009 Jul
TI  - Signalling strategies for osteogenic differentiation of human umbilical cord 
      mesenchymal stromal cells for 3D bone tissue engineering.
PG  - 398-404
LID - 10.1002/term.176 [doi]
AB  - Human umbilical cord mesenchymal stromal cells (hUCMSCs) have recently shown the 
      capacity to differentiate into multiple cell lineages in all three embryonic germ 
      layers. The osteogenic differentiation of hUCMSCs in monolayer culture has been 
      reported, while the differentiation in three-dimensional biomaterials has not yet 
      been reported for tissue-engineering applications. Thus, the aim of this study 
      was to evaluate the feasibility of using hUCMSCs for bone tissue engineering. 
      hUCMSCs were cultured in poly(L-lactic acid) (PLLA) scaffolds in osteogenic 
      medium (OM) for 3 weeks, after which the scaffolds were exposed to several 
      different media, including the OM, a mineralization medium (MM) and the MM with 
      either 10 or 100 ng/ml insulin-like growth factor (IGF)-1. The osteogenic 
      differentiation was confirmed by the up-regulation of Runx2 and OCN, calcium 
      quantification and bone histology. Switching from the OM to the MM promoted 
      collagen synthesis and calcium content per cell, while continuing in the OM 
      retained more cells in the constructs and promoted higher osteogenic gene 
      expression. The addition of IGF-1 into the MM had no effect on cell 
      proliferation, differentiation and matrix synthesis. In conclusion, hUCMSCs show 
      significant potential for bone tissue engineering and culturing in the OM 
      throughout the entire period is beneficial for osteogenic differentiation of 
      these cells.
FAU - Wang, Limin
AU  - Wang L
AD  - Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 
      48109, USA.
FAU - Singh, Milind
AU  - Singh M
FAU - Bonewald, Lynda F
AU  - Bonewald LF
FAU - Detamore, Michael S
AU  - Detamore MS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Culture Media)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - Bone and Bones/pathology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Culture Media/pharmacology
MH  - Humans
MH  - Insulin-Like Growth Factor I/metabolism
MH  - Lactic Acid/chemistry
MH  - Mesenchymal Stem Cells/*cytology
MH  - *Osteogenesis
MH  - Polyesters
MH  - Polymers/chemistry
MH  - Signal Transduction
MH  - Stem Cells/cytology
MH  - Stromal Cells/*cytology
MH  - Tissue Engineering/*methods
MH  - Umbilical Veins/*cytology
EDAT- 2009/05/13 09:00
MHDA- 2009/11/17 06:00
CRDT- 2009/05/13 09:00
PHST- 2009/05/13 09:00 [entrez]
PHST- 2009/05/13 09:00 [pubmed]
PHST- 2009/11/17 06:00 [medline]
AID - 10.1002/term.176 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2009 Jul;3(5):398-404. doi: 10.1002/term.176.