PMID- 19435617
OWN - NLM
STAT- MEDLINE
DCOM- 20091201
LR  - 20211020
IS  - 1878-7568 (Electronic)
IS  - 1742-7061 (Print)
IS  - 1742-7061 (Linking)
VI  - 5
IP  - 8
DP  - 2009 Oct
TI  - Reverse micelle-mediated synthesis of calcium phosphate nanocarriers for 
      controlled release of bovine serum albumin.
PG  - 3112-21
LID - 10.1016/j.actbio.2009.04.031 [doi]
AB  - Calcium phosphate (CaP) nanoparticles with a calcium to phosphorus (Ca:P) molar 
      ratio of 1.5:1 were synthesized using reverse microemulsion. Ca(NO(3))(2).4H(2)O 
      and H(3)PO(4) were used as the aqueous phase, cyclohexane as the organic phase 
      and poly(oxyethylene)(12) nonylphenol ether (NP-12) as the surfactant. Depending 
      on the calcination temperature between 600 and 800 degrees C, CaP nanoparticle 
      showed different phases of calcium-deficient hydroxyapatite (CDHA) and 
      beta-tricalcium phosphate (beta-TCP), particle size between 48 and 69 nm, and a 
      BET specific average surface area between 73 and 57 m(2)g(-1). Bovine serum 
      albumin (BSA) was used as a model protein to study loading and release behavior. 
      The adsorptive property of BSA was investigated by the change in BET surface area 
      of these nanoparticles and the pH of the suspension. At pH 7.5, the maximum 
      amount of BSA was adsorbed onto CaP nanoparticle. The release kinetics of BSA 
      showed a gradual time-dependent increase in pH 4.0 and 6.0 buffer solutions. 
      However, the amount of protein released was significantly smaller at pH 7.2. The 
      BSA release rate also varied depending on the presence of different phases of 
      CaPs in the system, beta-TCP or CDHA. These results suggest that the BSA protein 
      release rate can be controlled by changing the particle size, surface area and 
      phase composition of the CaP nanocarriers.
FAU - Dasgupta, Sudip
AU  - Dasgupta S
AD  - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and 
      Materials Engineering, Washington State University, Pullman, WA 99164, USA.
FAU - Bandyopadhyay, Amit
AU  - Bandyopadhyay A
FAU - Bose, Susmita
AU  - Bose S
LA  - eng
GR  - R01 EB007351/EB/NIBIB NIH HHS/United States
GR  - R01 EB007351-01A1/EB/NIBIB NIH HHS/United States
GR  - R01-EB-007351/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20090504
PL  - England
TA  - Acta Biomater
JT  - Acta biomaterialia
JID - 101233144
RN  - 0 (Biocompatible Materials)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Colloids)
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Micelles)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 97Z1WI3NDX (calcium phosphate)
SB  - IM
MH  - Absorption
MH  - Biocompatible Materials/*chemistry
MH  - Body Fluids/*chemistry
MH  - Calcium Phosphates/*chemistry
MH  - Colloids/chemistry
MH  - Crystallization/methods
MH  - Delayed-Action Preparations/*chemistry
MH  - Diffusion
MH  - Materials Testing
MH  - Micelles
MH  - Nanostructures/*chemistry/ultrastructure
MH  - Particle Size
MH  - Porosity
MH  - Serum Albumin, Bovine/administration & dosage/*chemistry
PMC - PMC2825089
MID - NIHMS164466
EDAT- 2009/05/14 09:00
MHDA- 2009/12/16 06:00
PMCR- 2010/10/01
CRDT- 2009/05/14 09:00
PHST- 2008/10/21 00:00 [received]
PHST- 2009/04/14 00:00 [revised]
PHST- 2009/04/24 00:00 [accepted]
PHST- 2009/05/14 09:00 [entrez]
PHST- 2009/05/14 09:00 [pubmed]
PHST- 2009/12/16 06:00 [medline]
PHST- 2010/10/01 00:00 [pmc-release]
AID - S1742-7061(09)00195-0 [pii]
AID - 10.1016/j.actbio.2009.04.031 [doi]
PST - ppublish
SO  - Acta Biomater. 2009 Oct;5(8):3112-21. doi: 10.1016/j.actbio.2009.04.031. Epub 
      2009 May 4.