PMID- 19436054
OWN - NLM
STAT- MEDLINE
DCOM- 20090724
LR  - 20211020
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 114
IP  - 1
DP  - 2009 Jul 2
TI  - HIV-1-infected dendritic cells show 2 phases of gene expression changes, with 
      lysosomal enzyme activity decreased during the second phase.
PG  - 85-94
LID - 10.1182/blood-2008-12-194845 [doi]
AB  - Dendritic cells (DCs) play a key role in the pathogenesis of HIV infection. HIV 
      interacts with these cells through 2 pathways in 2 temporal phases, initially via 
      endocytosis and then via de novo replication. Here the transcriptional response 
      of human DCs to HIV-1 was studied in these phases and at different stages of the 
      virus replication cycle using purified HIV-1 envelope proteins, and inactivated 
      and viable HIV-1. No differential gene expression was detected in response to 
      envelope. However, more than 100 genes were differentially expressed in response 
      to entry of viable and inactivated HIV-1 in the first phase. A completely 
      different set of genes was differentially expressed in the second phase, 
      predominantly in response to viable HIV-1, including up-regulation of immune 
      regulation genes, whereas genes encoding lysosomal enzymes were down-regulated. 
      Cathepsins B, C, S, and Z RNA and protein decreased, whereas cathepsin L was 
      increased, probably reflecting a concomitant decrease in cystatin C. The net 
      effect was markedly diminished cathepsin activity likely to result in enhanced 
      HIV-1 survival and transfer to contacting T lymphocytes but decreased HIV-1 
      antigen processing and presentation to these T cells.
FAU - Harman, Andrew N
AU  - Harman AN
AD  - Centre for Virus Research, Westmead Millennium Institute, Sydney, Australia.
FAU - Kraus, Marianne
AU  - Kraus M
FAU - Bye, Chris R
AU  - Bye CR
FAU - Byth, Karen
AU  - Byth K
FAU - Turville, Stuart G
AU  - Turville SG
FAU - Tang, Owen
AU  - Tang O
FAU - Mercier, Sarah K
AU  - Mercier SK
FAU - Nasr, Najla
AU  - Nasr N
FAU - Stern, Josh L
AU  - Stern JL
FAU - Slobedman, Barry
AU  - Slobedman B
FAU - Driessen, Christoph
AU  - Driessen C
FAU - Cunningham, Anthony L
AU  - Cunningham AL
LA  - eng
GR  - R01 DE015512/DE/NIDCR NIH HHS/United States
GR  - R01 GM099705/GM/NIGMS NIH HHS/United States
GR  - R01 DE015512-01/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20090512
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Cystatins)
RN  - 0 (DNA Primers)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (gp120 protein, Human immunodeficiency virus 1)
RN  - EC 3.4.- (Cathepsins)
SB  - IM
MH  - Antigen Presentation
MH  - Base Sequence
MH  - Cathepsins/genetics/metabolism
MH  - Cystatins/genetics/metabolism
MH  - DNA Primers/genetics
MH  - Dendritic Cells/immunology/metabolism/*virology
MH  - Gene Expression
MH  - Genes, Viral
MH  - HIV Envelope Protein gp120/physiology
MH  - HIV-1/*genetics/*pathogenicity/physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Kinetics
MH  - Lysosomes/enzymology/genetics
MH  - Models, Biological
MH  - Oligonucleotide Array Sequence Analysis
MH  - T-Lymphocytes/virology
MH  - Virus Internalization
MH  - Virus Replication
PMC - PMC2710958
EDAT- 2009/05/14 09:00
MHDA- 2009/07/25 09:00
PMCR- 2010/07/02
CRDT- 2009/05/14 09:00
PHST- 2009/05/14 09:00 [entrez]
PHST- 2009/05/14 09:00 [pubmed]
PHST- 2009/07/25 09:00 [medline]
PHST- 2010/07/02 00:00 [pmc-release]
AID - S0006-4971(20)37140-8 [pii]
AID - 2008/194845 [pii]
AID - 10.1182/blood-2008-12-194845 [doi]
PST - ppublish
SO  - Blood. 2009 Jul 2;114(1):85-94. doi: 10.1182/blood-2008-12-194845. Epub 2009 May 
      12.