PMID- 19437434
OWN - NLM
STAT- MEDLINE
DCOM- 20100412
LR  - 20191210
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 92
IP  - 4
DP  - 2010 Mar 15
TI  - Cartilage engineering using cell-derived extracellular matrix scaffold in vitro.
PG  - 1567-77
LID - 10.1002/jbm.a.32419 [doi]
AB  - A cell-derived extracellular matrix (ECM) scaffold was constructed using cultured 
      porcine chondrocytes via a freeze-drying method, and its ability to promote 
      cartilage formation was evaluated in vitro. Scanning electron microscope (SEM) 
      revealed that the scaffold had highly uniform porous microstructure. Then, rabbit 
      chondrocytes were seeded dynamically on ECM scaffold and cultured for 2 days, 1, 
      2, and 4 weeks in vitro for analysis. Polyglycolic acid (PGA) scaffold was used 
      as a control. On gross observation of neocartilage tissue, a silvery white 
      cartilage-like tissue was observed after 1 week of culture in ECM scaffold, while 
      similar morphology was seen only after 4 weeks in PGA scaffold. The volume of 
      neocartilage-like tissue was significantly increased in both ECM and PGA groups. 
      The compressive strength was gradually increased with time in ECM group, while 
      gradually decreased in PGA group. DNA, glycosaminoglycan (GAG) and collagen 
      contents also increased gradually with time in both groups, but showed more 
      significant increase in ECM group. Histological staining for GAG (Safranin O 
      staining) and type II collagen (immunohistochemistry) showed sustained 
      accumulation of ECM molecules with time, which gradually and uniformly filled 
      porous space in ECM scaffold. On the contrary, they accumulated only at the 
      peripheral area of PGA scaffold. These results suggest that a novel cell-derived 
      ECM scaffold can provide a promising environment for generating a high quality 
      cartilage in vitro.
CI  - (c) 2009 Wiley Periodicals, Inc.
FAU - Jin, Cheng Zhe
AU  - Jin CZ
AD  - Cell Therapy Center, Ajou University School of Medicine, Suwon, Gyeonggi, Korea.
FAU - Choi, Byung Hyune
AU  - Choi BH
FAU - Park, So Ra
AU  - Park SR
FAU - Min, Byoung-Hyun
AU  - Min BH
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Biocompatible Materials)
RN  - 0 (Collagen Type II)
RN  - 0 (Proteoglycans)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - *Cartilage/cytology/metabolism
MH  - Cell Shape
MH  - Cells, Cultured
MH  - Chondrocytes/cytology/metabolism
MH  - Collagen Type II/metabolism
MH  - Compressive Strength
MH  - Extracellular Matrix/*chemistry
MH  - Materials Testing
MH  - Microscopy, Electron, Scanning
MH  - Porosity
MH  - Proteoglycans/chemistry
MH  - Rabbits
MH  - Stress, Mechanical
MH  - Swine
MH  - Tissue Engineering/instrumentation/*methods
MH  - Tissue Scaffolds/*chemistry
EDAT- 2009/05/14 09:00
MHDA- 2010/04/13 06:00
CRDT- 2009/05/14 09:00
PHST- 2009/05/14 09:00 [entrez]
PHST- 2009/05/14 09:00 [pubmed]
PHST- 2010/04/13 06:00 [medline]
AID - 10.1002/jbm.a.32419 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2010 Mar 15;92(4):1567-77. doi: 10.1002/jbm.a.32419.