PMID- 19438950
OWN - NLM
STAT- MEDLINE
DCOM- 20110922
LR  - 20161125
IS  - 1708-8208 (Electronic)
IS  - 1523-0899 (Linking)
VI  - 13
IP  - 2
DP  - 2011 Jun
TI  - The influence of bone type on the gene expression in normal bone and at the 
      bone-implant interface: experiments in animal model.
PG  - 146-56
LID - 10.1111/j.1708-8208.2009.00195.x [doi]
AB  - BACKGROUND: Studies on the biological processes in different bone types and the 
      reaction of different bone types to biomaterials are often hindered because of 
      the difficulties in sampling procedures and lack of sensitive techniques. 
      PURPOSE: The purpose was to assess the suitability of quantitative polymerase 
      chain reaction (qPCR) for investigation of the biological differences between 
      cortical and trabecular bone types and their responses to biomaterials. MATERIALS 
      AND METHODS: Gene expression of selected markers in rat bone samples from 
      different locations was evaluated. Samples were harvested by trephines from the 
      trabecular femoral epiphysis, cortico-trabecular proximal tibial metaphysic, and 
      the cortical distal tibial metaphysis. Gene expression was also evaluated at the 
      surfaces of anodically oxidized implants retrieved from cortical and trabecular 
      sites after 3 days of implantation. mRNA in the bone samples and in the tissue 
      associated with the implant surfaces was extracted and quantified using qPCR. 
      Tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), alkaline phosphatase 
      (ALP), osteocalcin (OC), tartrate-resistant acid phosphatase (TRAP), cathepsin K 
      (CATK), and 18S ribosomal subunits (18S) were analyzed. RESULTS: In the bone 
      samples, higher expression of ALP, OC, TRAP, and CATK was found in femoral 
      epiphysis compared to proximal or distal tibial metaphysis, indicating a higher 
      turnover in the trabecular bone. On the other hand, TNF-alpha and IL-1beta showed higher 
      expression in both tibia sites compared with the femur site, which suggests 
      higher inflammatory potential in the cortical bone. In response to the oxidized 
      implants trabecular bone expressed a higher level of IL-1beta, whereas the implants 
      in cortical bone were associated with higher expression of ALP and OC. 
      CONCLUSION: There are biological differences between cortical and trabecular bone 
      types, both in the normal steady-state condition and in response to biomaterials. 
      Such differences can be characterized and discriminated quantitatively using a 
      sensitive technique such as qPCR.
CI  - (c) 2009 Wiley Periodicals, Inc.
FAU - Omar, Omar
AU  - Omar O
AD  - Department of Biomaterials, Sahlgrenska Academy at University of Goteborg, 
      Sweden. omar.omar@biomaterials.gu.se
FAU - Suska, Felicia
AU  - Suska F
FAU - Lenneras, Maria
AU  - Lenneras M
FAU - Zoric, Neven
AU  - Zoric N
FAU - Svensson, Sara
AU  - Svensson S
FAU - Hall, Jan
AU  - Hall J
FAU - Emanuelsson, Lena
AU  - Emanuelsson L
FAU - Nannmark, Ulf
AU  - Nannmark U
FAU - Thomsen, Peter
AU  - Thomsen P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Clin Implant Dent Relat Res
JT  - Clinical implant dentistry and related research
JID - 100888977
RN  - 0 (Biocompatible Materials)
RN  - 0 (Dental Implants)
RN  - 0 (Dental Materials)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Isoenzymes)
RN  - 0 (RNA, Ribosomal, 18S)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 104982-03-8 (Osteocalcin)
RN  - D1JT611TNE (Titanium)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.1.3.2 (Acid Phosphatase)
RN  - EC 3.1.3.2 (Tartrate-Resistant Acid Phosphatase)
RN  - EC 3.4.22.38 (Cathepsin K)
RN  - EC 3.4.22.38 (Ctsk protein, rat)
SB  - IM
MH  - Acid Phosphatase/analysis
MH  - Alkaline Phosphatase/analysis
MH  - Animals
MH  - Biocompatible Materials/*chemistry
MH  - Bone Density/physiology
MH  - Bone Remodeling/physiology
MH  - Bone Resorption/metabolism
MH  - Bone and Bones/anatomy & histology/*metabolism
MH  - Cathepsin K/analysis
MH  - *Dental Implants
MH  - Dental Materials/chemistry
MH  - Epiphyses/anatomy & histology/metabolism
MH  - Female
MH  - Femur/anatomy & histology/metabolism
MH  - Gene Expression/genetics
MH  - Inflammation Mediators/analysis
MH  - Interleukin-1beta/analysis
MH  - Isoenzymes/analysis
MH  - Models, Animal
MH  - Osteocalcin/analysis
MH  - Osteogenesis/physiology
MH  - Polymerase Chain Reaction
MH  - RNA, Ribosomal, 18S/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Tartrate-Resistant Acid Phosphatase
MH  - Titanium/chemistry
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 2009/05/15 09:00
MHDA- 2011/09/23 06:00
CRDT- 2009/05/15 09:00
PHST- 2009/05/15 09:00 [entrez]
PHST- 2009/05/15 09:00 [pubmed]
PHST- 2011/09/23 06:00 [medline]
AID - CID195 [pii]
AID - 10.1111/j.1708-8208.2009.00195.x [doi]
PST - ppublish
SO  - Clin Implant Dent Relat Res. 2011 Jun;13(2):146-56. doi: 
      10.1111/j.1708-8208.2009.00195.x.