PMID- 19439119
OWN - NLM
STAT- MEDLINE
DCOM- 20100511
LR  - 20171116
IS  - 1003-0603 (Print)
IS  - 1003-0603 (Linking)
VI  - 21
IP  - 5
DP  - 2009 May
TI  - [The influence of hypoxia inducible factor-1 alpha expression on apoptosis and 
      proliferation of T lymphocyte in the Peyer's patches after burn with delayed 
      fluid resuscitation in rats at high altitude].
PG  - 296-9
AB  - OBJECTIVE: To explore the influence of hypoxia inducible factor-1 alpha (HIF-1 
      alpha) expression on the apoptosis and proliferation of T lymphocyte after severe 
      burn with delayed fluid resuscitation at different altitude in rats. METHODS: One 
      hundred and thirty-two Wistar rats were subjected to burn injury [30% total body 
      surface area (TBSA), III degree], at 1,517 metres and 3,848 metres above sea 
      level, and they were randomly divided into three groups: delayed fluid 
      resuscitation group (DFR, n=30 at each altitude), immediate fluid resuscitation 
      group (IFR, n=30 at each altitude), and sham group (SG, n=6). The Peyer's patches 
      were harvested from the ileum of rats at 6, 12, 24, 48 and 72 hours after burn 
      respectively. The levels of HIF-1 alpha, CD3(+) and the rate of apoptosis in 
      Peyer's patches were detected by terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL), tissue microarray technology and 
      immunohistochemistry. RESULTS: The apoptosis rate in Peyer's patches was higher 
      in DFR group than that in IFR group. The increase in HIF-1 alpha expression was 
      observed mainly on cell nucleus in T lymphocytes. The expression levels of HIF-1 
      alpha in Peyer's patches were much higher in DFR group and IFR group than those 
      in SG, and they were higher at 3 848 metres than those at 1 517 metres, and also 
      higher in DFR group compared with IFR group (all P<0.05). The decrease in CD3(+) 
      expression was observed mainly on cell membrane in T lymphocytes. The expression 
      levels of CD3(+) in Peyer's patches were much higher in DFR group and IFR group 
      than those in SG group, and the trough value appeared at 12 hours after burn (all 
      P<0.05). CONCLUSION: High expression of HIF-1 alpha may be responsible for the 
      high apoptosis rate and decrease of T lymphocyte number in Peyer's patches after 
      severe burn with delayed fluid resuscitation in rats at high altitude.
FAU - Ma, Ming
AU  - Ma M
AD  - Lanzhou General Hospital of Lanzhou Military Region, Lanzhou 730050, Gansu, 
      China.
FAU - Zhang, Cheng
AU  - Zhang C
FAU - Liu, Yi
AU  - Liu Y
FAU - Zhou, Wen-jun
AU  - Zhou WJ
FAU - Zhang, Shi-fan
AU  - Zhang SF
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue
JT  - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo 
      weizhongbing jijiuyixue
JID - 9887521
RN  - 0 (CD3 Complex)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
SB  - IM
MH  - Altitude
MH  - Animals
MH  - Apoptosis
MH  - Burns/immunology/*metabolism/therapy
MH  - CD3 Complex/metabolism
MH  - Cell Proliferation
MH  - Disease Models, Animal
MH  - Female
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Male
MH  - Peyer's Patches/immunology/*metabolism/pathology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Wistar
MH  - Resuscitation
MH  - T-Lymphocytes/*immunology/pathology
EDAT- 2009/05/15 09:00
MHDA- 2010/05/12 06:00
CRDT- 2009/05/15 09:00
PHST- 2009/05/15 09:00 [entrez]
PHST- 2009/05/15 09:00 [pubmed]
PHST- 2010/05/12 06:00 [medline]
PST - ppublish
SO  - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2009 May;21(5):296-9.